Measurement of lysophospholipase D/autotaxin activity in human serum samples

Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing. LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate. It was...

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Published inClinical biochemistry Vol. 40; no. 3; pp. 274 - 277
Main Authors Nakamura, Kazuhiro, Ohkawa, Ryunosuke, Okubo, Shigeo, Tozuka, Minoru, Okada, Mitsumasa, Aoki, Shinya, Aoki, Junken, Arai, Hiroyuki, Ikeda, Hitoshi, Yatomi, Yutaka
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.02.2007
Subjects
Autotaxin
Clinical Laboratory Techniques
Female
Humans
Lysophosphatidic acid
Lysophosphatidylcholine
Lysophospholipase D
Male
Multienzyme Complexes - blood
Phosphodiesterase I - blood
Phosphoric Diester Hydrolases - blood
Plasma
Pyrophosphatases - blood
Sensitivity and Specificity
Sex Factors
Plasma
Lysophospholipase D
Lysophosphatidic acid
Autotaxin
Lysophosphatidylcholine
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Summary:Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing. LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate. It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2006.10.009