Synthesis and characterization of silver nanoparticles using Bacillus amyloliquefaciens and Bacillus subtilis to control filarial vector Culex pipiens pallens and its antimicrobial activity

• Published in: Artificial cells, nanomedicine, and biotechnology Vol. 45; no. 7; pp. 1369 - 1378
• Main Authors: Fouad, Hatem, Hongjie, Li, Yanmei, Ding, Baoting, Yu, El-Shakh, Ahmed, Abbas, Ghulam, Jianchu, Mo
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.11.2017; Taylor & Francis Ltd
• Subjects: Animals; Anti-Bacterial Agents - biosynthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antimicrobial activity; Antimicrobial agents; Bacillus amyloliquefaciens; Bacillus amyloliquefaciens - metabolism; Bacillus amyloliquefaciens - physiology; Bacillus subtilis; Bacillus subtilis - metabolism; Bacillus subtilis - physiology; Bacteria; Biosynthesis; Culex; Culex pipiens; Culex pipiens pallens; Diptera; Insecticides - chemistry; Insecticides - metabolism; Insecticides - pharmacology; Larva; Larvae; Metal Nanoparticles; Microorganisms; Mosquitoes; Nanoparticles; Nanotechnology; Pesticides; Pupa; Pupae; Silver; Silver - chemistry; Silver - metabolism; Silver - pharmacology; Spectrophotometry; vector mosquito control; Nanoparticles; antibacterial activity; Bacillus amyloliquefaciens; vector mosquito control; Bacillus subtilis; Culex pipiens pallens
• ISSN: 2169-1401; 2169-141X
• DOI: 10.1080/21691401.2016.1241793

Culex pipiens pallens are the most common mosquito's vector in Asia. In order to protect the people from diseases, the anti-mosquito population is necessary that uses safe and new bio-pesticides such as bacteria-AgNPs. In our report, we used two kinds of bacteria to synthesize silver nanoparticles to examine the toxic effect on the larvae and pupae of Cx. pipiens pallens and also used as antimicrobial activity. The biosynthesis of AgNPs and its characterization was carried out by UV-Vis spectrophotometry, FTIR, TEM, SEM, and EDX. The larvicidal and pupicidal assays revealed that the lethal concentration LC 50 values of Bacillus amyloliquefaciens-AgNPs were 0.72 ppm (I), 0.73 ppm (II), 0.69 ppm (III), 1.16 ppm (IV), and 4.18 (Pupae), while LC 50 values of Bacillus subtilis-AgNPs were 0.60 ppm (I), 0.62 ppm (II), 0.21 ppm (III), 0.28 ppm (IV), and 3.46 ppm (Pupae) after 72 h of exposure. Antibacterial activity test of AgNPs reveals better results against rice pathogenic bacteria than bacteria alone. Thus, bacteria-mediated silver nanoparticles have a rapid effect on vector mosquito and microbial pathogen suggesting savings of energy and resources. Hence, bacteria-AgNPs may be used in the future as an effective weapon to control vector mosquito and harmful bacteria.