Selective Interaction of Sugarcane eIF4E with VPgs from Sugarcane Mosaic Pathogens

• Published in: Viruses Vol. 13; no. 3; p. 518
• Main Authors: Yang, Zongtao, Dong, Meng, Cheng, Guangyuan, Liu, Shuxian, Zhang, Hai, Shang, Heyang, Zhou, Yingshuan, Huang, Guoqiang, Zhang, Muqing, Wang, Fengji, Xu, Jingsheng
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 22.03.2021; MDPI AG
• Subjects: eIF4E; Eukaryotic Initiation Factor-4E - metabolism; Plant Diseases - virology; Plant Proteins - metabolism; Potyvirus - metabolism; Protein Binding; SCMV; SCSMV; SrMV; Viral Proteins - metabolism; VPg; eIF4E; VPg; SCSMV; SCMV; SrMV
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v13030518

Eukaryotic translation initiation factor 4E (eIF4E) plays a key role in the infection of potyviruses in susceptible plants by interacting with viral genome-linked protein (VPg). Sugarcane ( spp.) production is threatened by mosaic disease caused by (SCMV), (SrMV), and (SCSMV). In this study, two and their isoform and 4E-binding protein coding genes were cloned from sugarcane cultivar and designated , , and , respectively. Real-time quantitative PCR analysis showed different expression profiles of these four genes upon SCMV challenge. A subcellular localization assay showed that SceIF4Ea, SceIF4Eb, SceIF(iso)4E, and ScnCBP were distributed in the nucleus and cytoplasm. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that SceIF4Ea/b and SceIF(iso)4E were selectively employed by different sugarcane mosaic pathogens, i.e., SCMV-VPg interacted with SceIF4Ea/b and SceIF(iso)4E, SrMV-VPg interacted with both SceIF4Eb and SceIF(iso)4E, and SCSMV-VPg interacted only with SceIF(iso)4E. Intriguingly, the BiFC assays, but not the Y2H assays, showed that ScnCBP interacted with the VPgs of SCMV, SrMV, and SCSMV. Competitive interaction assays showed that SCMV-VPg, SrMV-VPg, and SCMV-VPg did not compete with each other to interact with SceIF(iso)4E, and SceIF(iso)4E competed with SceIF4Eb to interact with SrMV-VPg but not SCMV-VPg. This study sheds light on the molecular mechanism of sugarcane mosaic pathogen infection of sugarcane plants and benefits sugarcane breeding against the sugarcane mosaic disease.