The effect of methyl jasmonate and light irradiation treatments on the stilbenoid biosynthetic pathway in Vitis vinifera cell suspension cultures

• Published in: Natural product research Vol. 32; no. 8; pp. 909 - 917
• Main Authors: Andi, Seyed Ali, Gholami, Mansour, Ford, Christopher M
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 18.04.2018; Taylor & Francis Ltd
• Subjects: Bioreactors; cell suspension cultures; Flavonoids; Irradiation; Light irradiation; MeJA elicitor; Metabolites; Methyl jasmonate; Pulp; Radiation; Stilbene; stilbenoids; Vitis vinifera; Vitis vinifera cv. Shahani; Vitis vinifera cv. Shahani; stilbenoids; MeJA elicitor; cell suspension cultures; light irradiation
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2017.1367782

Grape stilbenes are a well-known family of plant polyphenolics that have been confirmed to have many biological activities in relation to health benefits. In the present study, we investigated the effect of methyl jasmonate (MeJA) elicitor at four different concentrations (25, 50, 100 and 200 μM) in combination or not with high-level light irradiation (10,000 LUX) on a cell line obtained from the pulp of Vitis vinifera cv. Shahani. Our results showed that the stilbene synthesis pathway is inhibited by high-light conditions. A concentration of 50 μM MeJA was optimum for efficient production and high accumulation of total phenolics and total flavonoids as well as total stilbenoids. Furthermore, we showed that there is a significant negative correlation between the production of these metabolites and cell growth. These data provide valuable information for the future scale-up of cell cultures for the production of these very high value compounds in bioreactor system.