Immunopotential of Pasteurella multocida bivalent outer membrane protein-based vaccine entrapped in aluminum hydroxide nanoparticles

• Published in: Brazilian journal of microbiology Vol. 53; no. 4; pp. 2299 - 2307
• Main Authors: Pegu, Haladhar, Tamuly, Shantanu, Sharma, Rajeev Kumar, Borah, Probodh, Nath, Rita
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.12.2022; Springer Nature B.V
• Subjects: Aluminum; Aluminum Hydroxide; Animals; Bacterial Outer Membrane Proteins; Biomedical and Life Sciences; Commensals; Food Microbiology; Immunoglobulin G; Life Sciences; Medical Microbiology; Membrane proteins; Membranes; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Microparticles; Mycology; Nanoparticles; Opportunist infection; Outer membrane proteins; Pasteurella Infections - microbiology; Pasteurella multocida; Pasteurellosis; Proteins; Respiratory tract; Swine; Vaccines; Vaccines, Combined; Veterinary Microbiology - Short Communication; Nanoparticles; Vaccine; Outer membrane protein (OMP); Aluminum hydroxide; Pasteurella multocida
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-022-00795-1

Swine pasteurellosis is one of the most economically important diseases of pig caused by Pasteurella multocida ( P. multocida ) capsular types A and D. These organisms are commensals and opportunistic pathogens in the upper respiratory tract in pig. In the present study, we extracted whole outer membrane proteins (OMP) from P. multocida capsular types A and D and were mixed together in the ratio of 1:1 forming bivalent outer-membrane proteins. The bivalent OMP was adsorbed onto aluminum hydroxide nanoparticles. The size of aluminum hydroxide nanoparticles adsorbed outer membrane protein was found to be in the range of 125 to 130 nm. We observed that aluminum hydroxide nanoparticles adjuvanted bivalent OMP-based vaccine elicited quicker immune kinetics in terms of IgG response as compared to aluminum hydroxide microparticles adjuvanted bivalent bacterin vaccine against P. multocida capsular type A and D.