Role of Reactive Oxygen Species and Bcl-2 Family Proteins in TNF-α-Induced Apoptosis of Lymphocytes

• Published in: Bulletin of experimental biology and medicine Vol. 149; no. 2; pp. 180 - 183
• Main Authors: Ryazanceva, N. V., Novickiy, V. V., Zhukova, O. B., Biktasova, A. K., Chechina, O. E., Sazonova, E. V., Belkina, M. V., Chasovskih, N. Yu, Khaitova, Z. K.
• Format: Journal Article
• Language: English; Russian
• Published: Boston Springer US 01.08.2010; Springer
• Subjects: Adult; Apoptosis; Apoptosis - physiology; Biomedical and Life Sciences; Biomedicine; Caspases - metabolism; Cell Biology; Female; General Pathology and Pathophysiology; Humans; In Vitro Techniques; Internal Medicine; Laboratory Medicine; Lymphocytes; Lymphocytes - cytology; Lymphocytes - physiology; Male; Mitochondrial DNA; Pathology; Proto-Oncogene Proteins c-bcl-2 - metabolism; Reactive Oxygen Species - metabolism; Receptors, Tumor Necrosis Factor - metabolism; Statistics, Nonparametric; Tumor necrosis factor; Tumor Necrosis Factor-alpha - metabolism; apoptosis; Bcl-2 family proteins; tumor necrosis factor-α; mitochondria; reactive oxygen species
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-010-0902-7

We studied the in vitro apoptosis-inducing effect of recombinant TNF-α (rTNF-α) on blood lymphocytes from healthy donors. rTNF-α-induced apoptosis was accompanied by an increase in the number of cells with low mitochondrial transmembrane potential, increased intracellular content of reactive oxygen species, reduced content of Bcl-2, Bcl-xL, and Bax proteins, and elevated Bad content. The molecular mechanisms of these changes are discussed.