Genetic Analysis of MMP-2 and MMP-3 Polymorphisms Reveals the Association of MMP-3 rs522616 with Susceptibility to Persistent Apical Periodontitis

• Published in: Genes Vol. 16; no. 7; p. 758
• Main Authors: Olano-Dextre, Tulio L., Mateo-Castillo, José F., Nishiyama, Celso K., Santos, Carlos F., Garlet, Gustavo P., Silva, Renato M., Letra, Ariadne, das Neves, Lucimara Teixeira
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.06.2025; MDPI
• Subjects: Adult; Alleles; Bacterial infections; Bones; Case-Control Studies; Chronic illnesses; Endodontics; Enzymes; Extracellular matrix; Female; Gelatinase A; Gene Frequency; Genes; Genetic analysis; Genetic aspects; Genetic factors; Genetic polymorphisms; Genetic Predisposition to Disease; Genotype; Genotyping; Gum disease; Health aspects; Humans; Immune response; Male; Matrix metalloproteinase; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 3 - genetics; Metalloproteinase; Metalloproteins; Microorganisms; Middle Aged; Periapical Periodontitis - genetics; Periapical Periodontitis - pathology; Periodontitis; Pilot projects; Polymorphism, Single Nucleotide; Risk factors; Single-nucleotide polymorphism; Stromelysin 1; Brazil; endodontics; genetic predisposition; matrix metalloproteinase; persistent apical periodontitis; single nucleotide polymorphism
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes16070758

Objective: The aim of the present study was to investigate the association of polymorphic variants in matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-3 (MMP-3) genes and the occurrence of persistent apical periodontitis (PAP). Methods: DNA samples from 180 individuals were recruited and divided into two groups: Case group, 79 subjects with a history of PAP; control group, 101 healthy subjects. Five single nucleotide polymorphisms (SNPs) were selected for genotyping: rs243865, rs2285053, and rs2287074 in the MMP-2 gene, and rs679620 and rs522616 in the MMP-3 gene. The chi-square test or Exact Fisher (p < 0.05) and Odds Ratio (CI = 95%) were used to compare the frequencies of genotypes and alleles between the two groups. Results: A positive association was found for the MMP-3 rs522616 AG genotype (p = 0.025), the AG + GG genotypes (p = 0.015), and the G allele (p = 0.016) with PAP. Conclusions: The MMP-3 variant rs522616 was associated with PAP. Similar studies are needed analyzing other genes involved in extracellular matrix dynamics under inflammatory conditions to clarify the role of the genetic factors of PAP.