Pathogen translocation and histopathological lesions in an experimental model of Salmonella Dublin infection in calves receiving lactic acid bacteria and lactose supplements

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Vol. 13; no. 3; pp. 261 - 270
• Main Authors: Frizzo, Laureano S., Universidad Nacional del Litoral, Santa Fe, Argentina, Zbrun, Maria V., Universidad Nacional del Litoral, Santa Fe, Argentina, Soto, Lorena P., Universidad Nacional del Litoral, Santa Fe, Argentina, Bertozzi, Ezequiel, Universidad Nacional del Litoral, Santa Fe, Argentina, Sequeira, Gabriel J., Universidad Nacional del Litoral, Santa Fe, Argentina, Marti, Luis E., Universidad Nacional del Litoral, Santa Fe, Argentina, Signorini, Marcelo L., Universidad Nacional del Litoral, Santa Fe, Argentina, Rodriguez Armesto, Roberto, Universidad Nacional del Litoral, Santa Fe, Argentina, Rosmini, Marcelo R., Universidad Nacional del Litoral, Santa Fe, Argentina
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한수의학회 01.09.2012; The Korean Society of Veterinary Science
• Subjects: Administration, Oral; Animals; Animals, Newborn; CALVES; Cattle; Cattle Diseases - drug therapy; Cattle Diseases - microbiology; Cattle Diseases - pathology; Dietary Supplements - analysis; Feces - microbiology; LACTOBACILLUS; Lactobacillus - metabolism; LACTOSA; LACTOSE; Lactose - metabolism; Male; Original; Pediococcus - metabolism; PROBIOTICOS; PROBIOTICS; Probiotics - therapeutic use; PROBIOTIQUE; Salmonella Dublin challenge; Salmonella enterica - drug effects; Salmonella enterica - growth & development; Salmonella Infections, Animal - drug therapy; Salmonella Infections, Animal - microbiology; Salmonella Infections, Animal - pathology; TERNERO; Tissue Distribution; VEAU; 수의학; Calves; probiotic; lactose; Salmonella Dublin challenge; Lactobacillus
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2012.13.3.261

The purpose of this study was to evaluate the capacity of a lactic acid bacteria (LAB) inoculum to protect calves with or without lactose supplements against Salmonella Dublin infection by evaluating histopathological lesions and pathogen translocation. Fifteen calves were divided into three groups [control group (C-G), a group inoculated with LAB (LAB-G), and a group inoculated with LAB and given lactose supplements (L-LAB-G)] with five, six, and four animals, respectively. The inoculum, composed of Lactobacillus (L.) casei DSPV 318T, L. salivarius DSPV 315T, and Pediococcus acidilactici DSPV 006T, was administered with milk replacer. The LAB-G and L-LAB-G received a daily dose of 10∨9 CFU/kg body weight of each strain throughout the experiment. Lactose was provided to the L-LAB-G in closes of 100 g/day. Salmonella Dublin (2 × 10∨10 CFU) was orally administered to all animals on day 11 of the experiment. The microscopic lesion index values in target organs were 83%, 70%, and 64.3% (p less than 0.05) for the C-G, LAB-G, and L-LAB-G, respectively. Administration of the probiotic inoculum was not fully effective against infection caused by Salmonella. Although probiotic treatment was unable to delay the arrival of pathogen to target organs, it vas evident that the inoculum altered the response of animals against pathogen infection.