Bioguided isolation of N-malonyl-(+)-tryptophan from the fruit of Pithecellobium dulce (Roxb.) Benth. that showed high activity against Hymenolepis nana

Pithecellobium dulce is distributed in America and Asia where is widely used in traditional medicine. This study describes the bioguided fractionation of the methanol extract (ME) obtained from the P. dulce fruit that showed in vitro activity against Hymenolepis nana; Artemia salina assay was used t...

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Published inNatural product research Vol. 35; no. 4; pp. 593 - 599
Main Authors López-Angulo, Gabriela, Verdugo-Gaxiola, Sara Elizabeth, Montes-Avila, Julio, Díaz-Camacho, Sylvia Páz, Miranda-Soto, Valentín, Salazar-Salas, Nancy Yareli, Delgado-Vargas, Francisco
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 16.02.2021
Taylor & Francis Ltd
Subjects
Animals
Artemia
Artemia - drug effects
Chemical Fractionation
Fabaceae - chemistry
Fractionation
Fruit - chemistry
Fruits
Humans
Hymenolepis
Hymenolepis nana
Hymenolepis nana - drug effects
Hymenolepis nana - physiology
Metabolism
Paralysis
Parasites
parasitosis
Pithecellobium
Pithecellobium dulce
Plant Extracts - pharmacology
Praziquantel
Toxicity
treatment
Tryptophan
Tryptophan - isolation & purification
Tryptophan - pharmacology
parasitosis
treatment
Hymenolepis
Pithecellobium
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Summary:Pithecellobium dulce is distributed in America and Asia where is widely used in traditional medicine. This study describes the bioguided fractionation of the methanol extract (ME) obtained from the P. dulce fruit that showed in vitro activity against Hymenolepis nana; Artemia salina assay was used to determine toxicity; and the purified compound was computationally analysed to obtain its absorption-distribution-metabolism-excretion-and-toxicity properties (ADMET). The ME and its fractions were more active than praziquantel (PZQ), and the purified compound was characterized as N-malonyl-(+)-tryptophan (NMT). Parasites treated with NMT showed shorter paralysis and death times (5 and 7 min) than those treated with PZQ (15 and 30 min), both used at 20 mg/mL. Toxicity and ADMET prediction results supported the slight-hazardousness and efficacy of the assayed fractions/compound. This is the first report of the antiparasitary activity of both the P. dulce ME and NMT, showing their potential to treat human H. nana infections.
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ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2019.1590709