AGGF1 inhibits the expression of inflammatory mediators and promotes angiogenesis in dental pulp cells

• Published in: Clinical oral investigations Vol. 25; no. 2; pp. 581 - 592
• Main Authors: Shen, Song, Shang, Lingling, Liu, Hongrui, Liang, Qianyu, Liang, Wei, Ge, Shaohua
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2021; Springer Nature B.V
• Subjects: Angiogenesis; Angiogenic Proteins; Clinical trials; Cytokines; Dental pulp; Dental Pulp - metabolism; Dentistry; Enzyme-linked immunosorbent assay; Humans; Immunofluorescence; Immunohistochemistry; Inflammation; Inflammation Mediators; Interleukin 6; Interleukin 8; Lipopolysaccharides; Lipopolysaccharides - pharmacology; Medicine; Monocyte chemoattractant protein; Monocyte chemoattractant protein 1; Monocytes; NF-kappa B - metabolism; NF-κB protein; Nuclear transport; Original Article; Signal Transduction; Vascularization; Pulpitis; Angiogenic factor with G-patch and FHA domain 1; Angiogenesis; Inflammatory cytokines
• ISSN: 1432-6981; 1436-3771
• DOI: 10.1007/s00784-020-03498-9

Objectives To determine the role of angiogenic factor with G-patch and FHA domain 1 (AGGF1) in inflammatory response of human dental pulp cells (DPCs) and the underneath mechanism and to explore its role in angiogenesis. Materials and methods The expression of AGGF-1 in human healthy and inflammatory pulp tissues was detected by immunohistochemistry. RT-qPCR and Western blot were used to evaluate the expression of AGGF1 in DPCs stimulated by lipopolysaccharide (LPS). After AGGF1 was knocked down, the expression of LPS-induced inflammatory cytokines in DPCs was quantified by RT-qPCR and ELISA. Immunofluorescence and Western blot were used to assess the activation of NF-κB signaling. Inflammatory cytokines were detected by RT-qPCR and ELISA in DPCs pretreated with NF-κB pathway inhibitors before LPS stimulation, and then the effect of AGGF1 on angiogenesis was also evaluated. Results AGGF1 expression increased in inflammatory dental pulp tissues. In DPCs stimulated by LPS, AGGF1 was upregulated in a dose-dependent manner ( P < 0.05). In AGGF1 knockdown cells, the expression of IL-6, IL-8, and monocyte chemoattractant protein-1 (MCP-1/CCL-2) increased by LPS stimulation ( P < 0.001). Nuclear translocation of p65 was promoted, and the addition of NF-κB inhibitors inhibited the expression of inflammatory factors. Meanwhile, knockdown of AGGF1 inhibited vascularization. Conclusions AGGF1 inhibited the synthesis of inflammatory cytokines through NF-κB signaling pathway and promoted the angiogenesis of DPCs. Clinical relevance This study might shed light in the treatment of pulpitis and regeneration of dental pulp tissues; however, more clinical trials are required to validate these findings.