Rapid quantitative measurement of lung tissue phospholipids

A rapid procedure for the separation of phospholipids of lung tissue into acidic and nonacidic fractions by means of diethylaminoethyl cellulose acetate microcolumns is described. The fractions are then resolved into individual phospholipids by thin-layer chromatography and quantified by transmissio...

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Bibliographic Details
Published inJournal of lipid research Vol. 7; no. 4; pp. 570 - 574
Main Authors Gluck, L, Kulovich, M V, Brody, S J
Format Journal Article
LanguageEnglish
Published United States Elsevier 01.07.1966
Subjects
acidic
Animals
Chromatography
Chromatography, Thin Layer
DEAE cellulose columns
Densitometry
In Vitro Techniques
lung
Lung - analysis
nonacidic
phospholipids
Phospholipids - analysis
Rabbits
thin-layer chromatography
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Summary:A rapid procedure for the separation of phospholipids of lung tissue into acidic and nonacidic fractions by means of diethylaminoethyl cellulose acetate microcolumns is described. The fractions are then resolved into individual phospholipids by thin-layer chromatography and quantified by transmission densitometry.
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ISSN:0022-2275
DOI:10.1016/S0022-2275(20)39273-7