A highly selective and sensitive photoinduced electron transfer (PET) based HOCl fluorescent probe in water and its endogenous imaging in living cells

• Published in: Chemical communications (Cambridge, England) Vol. 52; no. 51; pp. 7982 - 7985
• Main Authors: Liang, Lijuan, Liu, Chang, Jiao, Xiaojie, Zhao, Liancheng, Zeng, Xianshun
• Format: Journal Article
• Language: English
• Published: CAMBRIDGE Royal Soc Chemistry 01.01.2016
• Subjects: Animals; Cell Line; Cells (biology); chemical compounds; Chemistry; Chemistry, Multidisciplinary; Conjugates; Electron transfer; Electron Transport; Fluorescence; fluorescence microscopes; fluorescent dyes; Fluorescent Dyes - chemistry; High resistance; Hydrogen-Ion Concentration; Hypochlorous Acid - chemistry; image analysis; Imaging; Mice; Microscopes; Microscopy, Fluorescence; Molecular Structure; Optical Imaging; photobleaching; Photochemical Processes; Physical Sciences; Real time; Science & Technology; Time Factors; Water - chemistry; CANCER-CELLS; REDOX CYCLE; MYELOPEROXIDASE; DUAL-EMISSION; SE-BODIPY; HYPOCHLOROUS ACID; DNA; MITOCHONDRIA; LIVE CELLS; HCLO
• ISSN: 1359-7345; 1364-548X; 1364-548X
• DOI: 10.1039/c6cc02603f

A probe based on the phenothiazine-acridine orange conjugate (Ptz-AO) has been designed and synthesized for the sensitive and selective detection of HOCl. Ptz-AO has excellent properties, including pH-independence of fluorescence, high resistance to photobleaching, and response in real time. The value of Ptz-AO was confirmed by exogenous, endogenous and the real-time imaging of HOCl in vitro using a fluorescence microscope. A probe based on the phenothiazine-acridine orange conjugate (Ptz-AO) has been designed and synthesized for the sensitive and selective detection of HOCl.