An integrated versatile lab-on-a-chip platform for the isolation and nucleic acid-based detection of pathogens

• Published in: Future science OA Vol. 3; no. 2; p. FSO177
• Main Authors: Sandetskaya, Natalia, Moos, Doreen, Pötter, Harald, Seifert, Stefan, Jenerowicz, Marcin, Becker, Holger, Zilch, Christian, Kuhlmeier, Dirk
• Format: Journal Article
• Language: English
• Published: England Future Science Ltd 01.05.2017
• Subjects: integrated sample preparation; lab-on-a-chip; LAMP; magnetic particles; PCR; integrated sample preparation; LAMP; lab-on-a-chip; PCR; magnetic particles
• ISSN: 2056-5623; 2056-5623
• DOI: 10.4155/fsoa-2016-0088

Processing of the samples in molecular diagnostics is complex and labor-intensive. An integrated and automated platform for sample preparation and nucleic acid-based detection can significantly relieve this burden for the users. We present a prototype of a versatile and integrated platform for the detection of pathogens in various liquid media. We describe a proof-of-concept for the integrated isolation of bacteria, cell lysis with optional DNA extraction, DNA amplification and detection in two different reactions, loop-mediated isothermal amplification and PCR, on a single microfluidic platform. The platform enables the transition from large sample volume to microfluidic format. The design and open interface enable its versatile application for various nucleic acid-based assays, from simple to complex setups. DNA-based detection of the pathogens in liquid samples demands many complex steps, various pieces of equipment, and experienced personnel. We developed and demonstrated a platform that can perform these steps automatically on a single instrument. This can enable molecular diagnostics in the places and situations where it was not possible before.