Biochemical Characterization of Dextranase from Arthrobacter oxydans and Its Cloning and Expression in Escherichia coli

• Published in: Food science and biotechnology Vol. 19; no. 3; pp. 757 - 762
• Main Authors: Lee, J.H., Chonnam National University, Gwangju, Republic of Korea, Nam, S.H., Jeonnam Agricultural Research and Extension Services, Naju, Republic of Korea, Park, H.J., Cosmax Ltd., Hwaseung, Republic of Korea, Kim, Y.M., Chonnam National University, Gwangju, Republic of Korea, Kim, N.H., Korean Minjok Leadership Academy, Hoeongseong, Republic of Korea, Kim, G.H., University of California-San Diego, La Jolla, CA, USA, Seo, E.S., Chonnam National University, Gwangju, Republic of Korea, Kang, S.S., Chonnam National University, Gwangju, Republic of Korea, Kim, D.M., Chonnam National University, Gwangju, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society of Food Science and Technology 01.06.2010; 한국식품과학회
• Subjects: Arthrobacter oxydans; AZUCAR; Chemistry; Chemistry and Materials Science; DEXTRANAS; DEXTRANASA; DEXTRANASE; DEXTRANE; DEXTRANS; Escherichia coli; expression; Food Science; Nutrition; Research Article; SUCRE; SUGAR; 식품과학; dextran; dextranase; expression; sugar
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-010-0106-y

Appreciably elevated levels of dextranase from Arthrobacter oxydans (AODex) isolated from sugar-cane farm soil was resulted from the culture on the Luria-Bertani (LB) medium containing 1%(w/v) soluble starch, glycerol, or dextran. The responsible gene (aodex) was cloned, its nucleotide sequence was determined, and expression of the encoded protein was achieved in Escherichia coli. An open reading frame was composed of 1,863 bp putatively encoding a 68.3 kDa protein. Recombinant A. oxydans dextranase (rAODex) was purified about 16 fold by nickel-nitrilotriacetic acid affinity column chromatography; K∧m value for dextran T2000 was 0.85 mg/mL (w/v). AODex treatment of stale sugar cane juice resulted in a yield of square and light-colored sugar crystals.