Parallel Analysis of 7 Food-borne Pathogens Using Capillary Electrophoresis-based Single-strand Conformation Polymorphism

• Published in: Food science and biotechnology Vol. 19; no. 6; pp. 1441 - 1447
• Main Authors: Kim, A.R., Ewha Womans University, Seoul, Republic of Korea, Oh, M.H., National Institute of Animal Science, RDA, Suwon, Republic of Korea, Seol, K.H., National Institute of Animal Science, RDA, Suwon, Republic of Korea, Shin, G.W., Pohang University of Science and Technology, Pohang, Republic of Korea, Jung, G.Y., Pohang University of Science and Technology, Pohang, Republic of Korea, Oh, S.S., Ewha Womans University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society of Food Science and Technology 01.12.2010; 한국식품과학회
• Subjects: Bacillus cereus; capillary electrophoresis-based single-strand conformation polymorphism; Chemistry; Chemistry and Materials Science; Colony-forming cells; Food Science; food-borne pathogen; multiplex polymerase chain reaction; Nutrition; PCR; Research Article; 식품과학; multiplex polymerase chain reaction (PCR); capillary electrophoresis-based single-strand conformation polymorphism (CESSCP); food-borne pathogen
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-010-0206-8

Capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) coupled with multiplex polymerase chain reaction (PCR) method was used for the detection of 7 pathogens associated with food-borne illness, including Salmonella enterica, Clostridium perfringens, Bacillus cereus, Listeria monocytogenes, Yersinia enterocolitica, Vibrio parahaemolyticus, and Escherichia coli O157:H7. The method was applied to model food systems, both of culture medium and cooked rice. The detection limit of individual microbes was in the range of 10¹-10³ CFU/g, and that of the mixture of 7 microbes was 10³ CFU/g in the cooked rice sample. This method allowed the detection and identification of all 7 food-borne pathogens within 5 hr without the requirement for enrichment steps.