Formulation of liposomes functionalized with Lotus lectin and effective in targeting highly proliferative cells

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 4; pp. 860 - 870
• Main Authors: Della Giovampaola, Cinzia, Capone, Antonietta, Ermini, Leonardo, Lupetti, Pietro, Vannuccini, Elisa, Finetti, Federica, Donnini, Sandra, Ziche, Marina, Magnani, Agnese, Leone, Gemma, Rossi, Claudio, Rosati, Floriana, Bonechi, Claudia
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2017
• Subjects: Animals; Binding Sites; Cell Line, Tumor; Cell Proliferation - drug effects; Chemistry, Pharmaceutical - methods; confocal laser scanning microscopy; Cytoplasm - drug effects; Doxorubicin; Doxorubicin - administration & dosage; Doxorubicin - chemistry; Drug delivery; Drug Delivery Systems - methods; drug therapy; Epitopes - administration & dosage; Epitopes - chemistry; Fabaceae - metabolism; fucose; Humans; lectins; Lectins - administration & dosage; Lectins - chemistry; Liposomes - administration & dosage; Liposomes - chemistry; Lotus lectin; Lotus tetragonolobus; lysosomes; Lysosomes - drug effects; melanoma; Melanoma, Experimental - drug therapy; Mice; NMR; polysaccharides; Targeted liposomes; DOPE; LTL; LTL-Dex-L; LTL-Dox-L; Drug delivery; Targeted liposomes; Dox; L; Lotus lectin; Doxorubicin; NMR; LTL-L; Dex; Dex-L; DOPC; Dox-L
• ISSN: 0304-4165; 1872-8006
• DOI: 10.1016/j.bbagen.2017.01.015

Liposomes, used to improve the therapeutic index of new and established drugs, have advanced with the insertion of active targeting. The lectin from Lotus tetragonolobus (LTL), which binds glycans containing alpha-1,2-linked fucose, reveals surface regionalized glycoepitopes in highly proliferative cells not detectable in normally growing cells. In contrast, other lectins localize the corresponding glycoepitopes all over the cell surface. LTL also proved able to penetrate the cells by an unconventional uptake mechanism. We used confocal laser microscopy to detect and localize LTL-positive glycoepitopes and lectin uptake in two cancer cell lines. We then constructed doxorubicin-loaded liposomes functionalized with LTL. Intracellular delivery of the drug was determined in vitro and in vivo by confocal and electron microscopy. We confirmed the specific localization of Lotus binding sites and the lectin uptake mechanism in the two cell lines and determined that LTL-functionalized liposomes loaded with doxorubicin greatly increased intracellular delivery of the drug, compared to unmodified doxorubicin-loaded liposomes. The LTL-Dox-L mechanism of entry and drug delivery was different to that of Dox-L and other liposomal preparations. LTL-Dox-L entered the cells one by one in tiny tubules that never fused with lysosomes. LTL-Dox-L injected in mice with melanoma specifically delivered loaded Dox to the cytoplasm of tumor cells. Liposome functionalization with LTL promises to broaden the therapeutic potential of liposomal doxorubicin treatment, decreasing non-specific toxicity. Doxorubicin-LTL functionalized liposomes promise to be useful in the development of new cancer chemotherapy protocols.