Evaluation of antibacterial activity of the bark and leaf extracts of Brosimum gaudichaudii Trécul against multidrug resistant strains

• Published in: Natural product research Vol. 31; no. 24; pp. 2931 - 2935
• Main Authors: Borges, Joelma da Costa, Perim, Michele Cezimbra, de Castro, Rodrigo Orlandini, Araújo, Thiago Antônio de Sousa, Peixoto Sobrinho, Tadeu José da Silva, da Silva, Ana Carolina Oliveira, Mariano, Sandra Maria Botelho, Carreiro, Solange Cristina, Pranchevicius, Maria Cristina da Silva
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 17.12.2017; Taylor & Francis Ltd
• Subjects: Agar; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibiotics; Antimicrobial activity; Antimicrobial agents; Bacteria; Bark; Bioactive compounds; Brazil; Brosimum; Diabetes mellitus; diabetic foot infection; Dilution; Drug Resistance, Multiple - drug effects; Gram-negative bacteria; Gram-Negative Bacteria - drug effects; Gram-positive bacteria; Gram-Positive Bacteria - drug effects; Leaves; Moraceae - chemistry; Multidrug resistance; Multidrug resistant bacteria; pharmacological potential, medicinal plants; Plant Bark - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Leaves - chemistry; Pseudomonas aeruginosa; Statistical analysis; Strains (organisms); Multidrug resistant bacteria; antimicrobial activity; pharmacological potential, medicinal plants; diabetic foot infection
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2017.1305379

Brosimum gaudichaudii Trécul., a plant that belongs to Moraceae family, is found throughout the Brazilian Cerrado. The antimicrobial activities of ethanolic bark and leaf extracts of B. gaudichaudii were tested against multiresistant bacteria isolated from diabetic foot infections (DFIs). Antimicrobial activity of the extracts was evaluated by agar disc diffusion (DD) and broth dilution (BD) methods. By BD method, bark (53.85, 45.83%) and leaf (42.31, 50.00%) extracts contained antimicrobial activity against both gram-negative and gram-positive bacteria. Increased antimicrobial activity was observed when bark and leaf extracts were tested against Staphylococcus aureus (63.64%) and Pseudomonas aeruginosa (66.67%). Statistical analyses of bark and leaf extract demonstrated antimicrobial activity against both gram-positive (p = 0.000) and gram-negative bacteria (p = 0.012). Extract of bark (p = 0.075) or leaf (p = 0.005) associated with ACA antibiotic showed antimicrobial activity against gram-positive bacteria. Our study suggests that the bark and leaf extracts contain bioactive compounds with antimicrobial activity against multidrug resistant strains.