Use of urinary 13,14, dihydro-15-keto-prostaglandin F2α (PGFM) concentrations to diagnose pregnancy and predict parturition in the giant panda (Ailuropoda melanolecua)

• Published in: PloS one Vol. 13; no. 5; p. e0195599
• Main Authors: Roberts, Beth M, Brown, Janine L, Kersey, David C, Snyder, Rebecca J, Durrant, Barbara S, Kouba, Andrew J
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.05.2018; Public Library of Science (PLoS)
• Subjects: Ailuropoda; Animal reproduction; Biology; Biology and Life Sciences; Conservation; Endocrinology; Enzyme immunoassay; Enzymes; Excretion; Gestation; Immunoassay; Low concentrations; Medicine and Health Sciences; Metabolism; Metabolites; Ovulation; Pandas; Parturition; Physiology; Pregnancy; Pseudopregnancy; Ultrasonic imaging; Urine; Veterinary medicine; Wildlife conservation; New York; Mississippi; California; United States > US; Tennessee; Oklahoma
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0195599

Pregnancy determination is difficult in the giant panda (Ailuropoda melanolecua), representing a challenge for ex situ conservation efforts. Research in other species experiencing pseudopregnancy indicates that urinary/fecal concentrations of 13,14, dihydro-15-keto-prostaglandin F2α (PGFM) can accurately determine pregnancy status. Our objective was to determine if urinary PGFM concentrations are associated with pregnancy status in the giant panda. Urinary PGFM concentrations were measured in female giant pandas (n = 4) throughout gestation (n = 6) and pseudopregnancy (n = 4) using a commercial enzyme immunoassay. Regardless of pregnancy status, PGFM excretion followed a predictable pattern: 1) baseline concentrations for 11-19 weeks following ovulation; 2) a modest, initial peak 14-36 days after the start of the secondary urinary progestagen rise; 3) a subsequent period of relatively low concentrations; and 4) a large, terminal peak at the end of the luteal phase. Pregnant profiles were distinguished by an earlier initial peak (P = 0.024), higher inter-peak concentrations (P < 0.001), and a larger terminal peak (P = 0.003) compared to pseudopregnancy profiles. Parturition occurred 23 to 25 days from the initial PGFM surge and within 24 hours of the start of the terminal increase. These pattern differences indicate that urinary PGFM monitoring can be used to predict pregnancy status and time parturition in the giant panda. Furthermore, this is the only species known to exhibit a significant PGFM increase during pseudopregnancy, suggesting a unique physiological mechanism for regulating the end of the luteal phase in the giant panda.