Complement activation by commercial allergen extracts of cereal grains

Commercial allergen extract preparations of whole cereal grains were examined in vitro for their reactivity with the human haemolytic complement cascade. Total haemolytic complement was consumed in a dose-dependent manner which did not correlate with the protein nitrogen content or bacterial endotox...

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Bibliographic Details
Published inClinical allergy Vol. 10; no. 4; pp. 395 - 404
Main Authors Olenchock, S A, Mull, J C, Major, P C
Format Journal Article
LanguageEnglish
Published England 01.07.1980
Subjects
Allergens - immunology
Chemotaxis, Leukocyte
Commerce
Complement Activation
Complement C1
Complement C2
Complement C3
Complement C4
Edetic Acid - pharmacology
Edible Grain
Endotoxins - pharmacology
Humans
Nitrogen
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Summary:Commercial allergen extract preparations of whole cereal grains were examined in vitro for their reactivity with the human haemolytic complement cascade. Total haemolytic complement was consumed in a dose-dependent manner which did not correlate with the protein nitrogen content or bacterial endotoxin contamination of the extracts. Alternative pathway activation was shown by C3 conversion in the presence of EGTA while classical complement components C1, C4 and C2 were also much decreased in serum which was free of detectable specific antibody. Very small amounts of allergen extract initiated the formation of complement-dependent factors which were chemotactic for human polymorphonuclear leucocytes. These data suggest a potential for complement activation in vivo by ingestion, inhalation, or injection of cereal grain products.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0009-9090
DOI:10.1111/j.1365-2222.1980.tb02122.x