Doxorubicin Conjugated to Glutathione Stabilized Gold Nanoparticles (Au-GSH-Dox) as an Effective Therapeutic Agent for Feline Injection-Site Sarcomas-Chick Embryo Chorioallantoic Membrane Study

• Published in: Molecules (Basel, Switzerland) Vol. 22; no. 2; p. 253
• Main Authors: Zabielska-Koczywąs, Katarzyna, Dolka, Izabella, Król, Magdalena, Żbikowski, Artur, Lewandowski, Wiktor, Mieczkowski, Józef, Wójcik, Michał, Lechowski, Roman
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08.02.2017; MDPI
• Subjects: Animals; Antibiotics, Antineoplastic - administration & dosage; Biomarkers; CAM assay; Cats; Cell Line, Tumor; Cell lines; Chemical compounds; Chemotherapy; Chick Embryo; Chorioallantoic membrane; Chorioallantoic Membrane - pathology; Cytotoxicity; Disease Models, Animal; Doxorubicin; Doxorubicin - administration & dosage; Embryos; feline injection-site sarcoma; Fibrosarcoma; Glutathione - chemistry; Gold; Gold - chemistry; gold nanoparticles; in vivo study; Injection; Injections, Intralesional; Ki-67; Membranes; Metal Nanoparticles - chemistry; Nanoparticles; Pharmacology; Radiation therapy; Sarcoma; Sarcoma - drug therapy; Sarcoma - metabolism; Sarcoma - pathology; Tumor Burden - drug effects; Tumors; Veterinary medicine; Ki-67; in vivo study; doxorubicin; CAM assay; gold nanoparticles; feline injection-site sarcoma
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules22020253

Feline injection-site sarcomas are malignant skin tumours with a high local recurrence rate, ranging from 14% to 28%. The treatment of feline injection-site sarcomas includes radical surgery, radiotherapy and/or chemotherapy. In our previous study it has been demonstrated that doxorubicin conjugated to glutathione-stabilized gold nanoparticles (Au-GSH-Dox) has higher cytotoxic effects than free doxorubicin for feline fibrosarcoma cell lines with high glycoprotein P activity (FFS1, FFS3). The aim of the present study was to assess the effectiveness of intratumoural injection of Au-GSH-Dox on the growth of tumours from the FFS1 and FFS3 cell lines on chick embryo chorioallantoic membrane. This model has been utilized both in human and veterinary medicine for preclinical oncological studies. The influence of intratumoural injections of Au-GSH-Dox, glutathione-stabilized gold nanoparticles and doxorubicin alone on the Ki-67 proliferation marker was also checked. We demonstrated that the volume ratio of tumours from the FFS1 and FFS3 cell lines was significantly ( < 0.01) decreased after a single intratumoural injection of Au-GSH-Dox, which confirms the positive results of in vitro studies and indicates that Au-GSH-Dox may be a potent new therapeutic agent for feline injection-site sarcomas.