Bovine pulmonary surfactant: chemical composition and physical properties

Bovine pulmonary surfactant was obtained by endotracheal lavage of lungs from newly slaughtered cows followed by differential centrifugation. Lipid extracts of bovine surfactant contained 3% neutral lipid, mainly as cholesterol and diacylglycerol and 97% phospholipid. Phosphatidylcholine (79%) and p...

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Bibliographic Details
Published inLipids Vol. 18; no. 8; pp. 522 - 529
Main Authors Yu, Sue, Harding, Paul G. R., Smith, Norman, Possmayer, Fred
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer‐Verlag 01.08.1983
Springer
Subjects
Analysis of complex biological substances
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Blood and biological fluids
Cattle - metabolism
Cholesterol - analysis
Fundamental and applied biological sciences. Psychology
Glycerides - analysis
Lipids - analysis
Phospholipids - analysis
Proteins - analysis
Pulmonary Surfactants - analysis
Pulmonary Surfactants - isolation & purification
Surface Properties
Vertebrata
Mammalia
Pulmonary surfactant
Chemical composition
Artiodactyla
Beef
Ungulata
Physicochemical properties
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Summary:Bovine pulmonary surfactant was obtained by endotracheal lavage of lungs from newly slaughtered cows followed by differential centrifugation. Lipid extracts of bovine surfactant contained 3% neutral lipid, mainly as cholesterol and diacylglycerol and 97% phospholipid. Phosphatidylcholine (79%) and phosphatidylglycerol (11%) accounted for most of the phospholipids with smaller amounts of phosphatidyl‐ethanolamine, phosphatidylinositol, lyso‐bis‐phosphatidic, acid and sphingomyelin. Fatty acid analysis revealed high levels of palmitate in phosphatidylcholine and to a lesser extent phosphatidylglycerol, but not in the other diacylphospholipids. Phosphatidylcholine was 53% disaturated and phosphatidylglycerol was 23% disaturated. Monoenoic species accounted for the major proportion of the remaining, lipid. The protein content was 10% as estimated by the Lowry procedure and 5% when determined by amino acid analysis. Extraction with chloroform/methanol removed ca. 90% of the protein but had no effect on the surfactant properties as evaluated by a pulsating bubble technique.
Bibliography:L
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Some of the results contained in this paper were presented at the 73rd Meeting of the AOCS in Toronto, May, 1982.
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0024-4201
1558-9307
DOI:10.1007/BF02535391