The pcz1 Gene, which Encodes a Zn(II)2Cys6 Protein, Is Involved in the Control of Growth, Conidiation, and Conidial Germination in the Filamentous Fungus Penicillium roqueforti

• Published in: PloS one Vol. 10; no. 3; p. e0120740
• Main Authors: Gil-Durán, Carlos, Rojas-Aedo, Juan F., Medina, Exequiel, Vaca, Inmaculada, García-Rico, Ramón O., Villagrán, Sebastián, Levicán, Gloria, Chávez, Renato
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.03.2015; Public Library of Science (PLoS)
• Subjects: Aspergillus; Aspergillus nidulans; Carbon; Carbon - metabolism; Carbon sources; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungi; Gene expression; Gene Expression Regulation, Fungal; Gene Silencing; Genes; Germination; Glucose; Kinases; Metabolism; Metabolites; Neurospora crassa; Penicillium; Penicillium - physiology; Penicillium chrysogenum; Penicillium roqueforti; Phenotype; Protein Subunits; Proteins; RNA Interference; RNA, Small Interfering - genetics; RNA-mediated interference; Saccharomyces cerevisiae; Yeast; Yeasts; Zinc; Chile
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0120740

Proteins containing Zn(II)(2)Cys(6) domains are exclusively found in fungi and yeasts. Genes encoding this class of proteins are broadly distributed in fungi, but few of them have been functionally characterized. In this work, we have characterized a gene from the filamentous fungus Penicillium roqueforti that encodes a Zn(II)(2)Cys(6) protein, whose function to date remains unknown. We have named this gene pcz1. We showed that the expression of pcz1 is negatively regulated in a P. roqueforti strain containing a dominant active Gαi protein, suggesting that pcz1 encodes a downstream effector that is negatively controlled by Gαi. More interestingly, the silencing of pcz1 in P. roqueforti using RNAi-silencing technology resulted in decreased apical growth, the promotion of conidial germination (even in the absence of a carbon source), and the strong repression of conidiation, concomitant with the downregulation of the genes of the central conidiation pathway brlA, abaA and wetA. A model for the participation of pcz1 in these physiological processes in P. roqueforti is proposed.