Developmental ability of human oocytes with or without birefringent spindles imaged by Polscope before insemination

• Published in: Human reproduction (Oxford) Vol. 16; no. 7; pp. 1464 - 1468
• Main Authors: Wang, W.H., Meng, L., Hackett, R.J., Keefe, D.L.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.07.2001
• Subjects: Adult; Biological and medical sciences; Birefringence; Blastocyst - physiology; Culture Techniques; embryo development; Embryo, Mammalian - physiology; Female; Fundamental and applied biological sciences. Psychology; human oocytes; Humans; Image Processing, Computer-Assisted; Mammalian female genital system; Meiosis; Microscopy, Polarization; Morphology. Physiology; Morula - physiology; Oocytes - physiology; Oocytes - ultrastructure; Polscope; Sperm Injections, Intracytoplasmic; Spindle Apparatus; spindles; Vertebrates: reproduction; embryo development; spindles; human oocytes; Polscope; Human; Embryonic development; Image analysis; Fertilization; Artificial insemination; Prediction; Assisted procreation; Female; Meiosis; Germinal cell; Computer aid; Oocyte
• ISSN: 0268-1161; 1460-2350; 1460-2350
• DOI: 10.1093/humrep/16.7.1464

BACKGROUND: Birefringent spindles imaged with the Polscope can predict fertilization rates after intracytoplasmic sperm injection (ICSI). The present study examined the development of human oocytes with or without birefringent spindles, imaged with the Polscope before ICSI. METHODS: Oocytes were obtained from stimulated ovaries of consenting patients undergoing oocyte retrieval for ICSI. Spindles were imaged with the Polscope combined with a computerized image analysis system. After imaging and ICSI, oocytes with or without spindles were cultured separately for examination of fertilization and embryo development. A total of 1544 oocytes from 136 cycles were examined with the Polscope and inseminated by ICSI. RESULTS: Spindles were imaged in 82% of oocytes. After ICSI, more oocytes (P < 0.05) with spindles (69.4%) fertilized normally, forming 2 pronuclei, than oocytes without spindles (62.9%). At day 3, more oocytes (P < 0.01) with spindles (66.3%) developed to 4–11 cell stages than oocytes without spindles (55.4%). Significantly more (P < 0.001) oocytes with spindles developed to morula and blastocyst by day 5 (51.1 versus 30.3%) and day 6 (53.2 versus 29.3%) compared with oocytes without spindles. CONCLUSIONS: The results indicate that the presence of a birefringent spindle in human oocytes can predict not only higher fertilization rate, but also higher embryo developmental competence.