Molecular cloning of rat intestinal mucin. Lack of conservation between mammalian species
We have prepared antisera to deglycosylated rat intestinal mucin and used it to obtain immunoreactive clones from a rat jejunum cDNA library. Four of these clones were sequenced, and all were found to be partial cDNAs that contained 18-base pair tandem repeats characteristic of a mucin. These cDNAs...
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Published in | The Journal of biological chemistry Vol. 266; no. 33; pp. 22733 - 22738 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
American Society for Biochemistry and Molecular Biology
25.11.1991
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Subjects | |
Online Access | Get full text |
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Summary: | We have prepared antisera to deglycosylated rat intestinal mucin and used it to obtain immunoreactive clones from a rat jejunum
cDNA library. Four of these clones were sequenced, and all were found to be partial cDNAs that contained 18-base pair tandem
repeats characteristic of a mucin. These cDNAs encoded a repetitive peptide with a consensus sequence of TTTPDV. Thus, they
bear little resemblance to either of the two human intestinal mucin cDNAs isolated previously (Gum, J. R., Byrd, J. C., Hicks,
J. W., Toribara, N. W., Lamport, D. T. A., and Kim, Y. S. (1989) J. Biol. Chem. 264, 6480-6487 and Gum, J. R., Hicks, J. W.,
Swallow, D. M., Lagace, R. E., Byrd, J. C., Lamport, D. T. A., Siddiki, B., and Kim, Y. S. (1990) Biochem. Biophys. Res. Commun.
171, 407-415). One of these rat mucin clones, designated RMUC 176, was chosen for further analysis. This clone recognized
a band of approximately 9 kilobases when used to probe RNA blots. A strong hybridization band was present using rat small
intestine and colon RNA but was not detectable when RNA isolated from heart, liver, or kidney was tested. The RMUC 176 clone
and the two previously isolated human intestinal mucin cDNA clones were used to probe blots prepared from BamHI-digested DNA
of various species. Here, the human probes detected fragments present only in human and chimpanzee DNA, whereas the RMUC 176
clone recognized fragments only in rat and mouse DNA. Thus, the repetitive portions of intestinal mucin genes are apparently
not well conserved between phylogenetically distant species. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(18)54629-9 |