transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica)

• Published in: Tree genetics & genomes Vol. 9; no. 2; pp. 557 - 571
• Main Authors: Kubisiak, T. L, Nelson, C. D, Staton, M. E, Zhebentyayeva, T, Smith, C, Olukolu, B. A, Fang, G.-C, Hebard, F. V, Anagnostakis, S, Wheeler, N, Sisco, P. H, Abbott, A. G, Sederoff, R. R
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.04.2013; Springer Nature B.V
• Subjects: 20th century; Biomedical and Life Sciences; Biotechnology; Blight; Castanea; Castanea dentata; Castanea mollissima; Cloning; Conservation; Cryphonectria parasitica; Disease resistance; expressed sequence tags; Fagaceae; Forestry; fungi; Gene mapping; genes; Genetic markers; Genetics; genome assembly; Genomes; Genomics; Homology; introgression; Life Sciences; linkage groups; loci; Original Paper; parents; Pathogens; peaches; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Polymorphism; Powdery mildew; progeny; Prunus; Prunus persica; Quantitative trait loci; Simple sequence repeats; Single-nucleotide polymorphism; Synteny; Timber; Tree Biology; Trees; North Carolina; United States > US; Comparative genomics; Disease resistance; spp; Fagaceae; Genetic map; Chestnut
• ISSN: 1614-2942; 1614-2950
• DOI: 10.1007/s11295-012-0579-3

The Chinese chestnut (Castanea mollissima) carries resistance to Cryphonectria parasitica, the fungal pathogen inciting chestnut blight. The pathogen, introduced from Asia, devastated the American chestnut (Castanea dentata) throughout its native range early in the twentieth century. A highly informative genetic map of Chinese chestnut was constructed to extend genomic studies in the Fagaceae and to aid the introgression of Chinese chestnut blight resistance genes into American chestnut. Two mapping populations were established with three Chinese chestnut parents, ‘Mahogany’, ‘Nanking’, and ‘Vanuxem’, totaling 337 progeny. The transcriptome-based genetic map was created with 329 simple sequence repeat and 1,064 single nucleotide polymorphism markers all derived from expressed sequence tag sequences. Genetic maps for each parent were developed and combined to establish 12 consensus linkage groups spanning 742 cM, providing the the most comprehensive genetic map for a Fagaceae species to date. Over 75 % of the mapped markers from the Chinese chestnut consensus genetic map were placed on the physical map using overgo hybridization, providing a fully integrated genetic and physical map resource for Castanea spp. About half (57 %) of the Chinese chestnut genetic map could be assigned to regions of segmental homology with 58 % of the peach (Prunus persica) genome assembly. A three quantitative trait loci (QTL) model for blight resistance was verified using the new genetic markers and an existing interspecies (C. mollissima × C. dentata) F₂ mapping population. Two of the blight resistance QTLs in chestnut shared synteny with two QTLs for powdery mildew resistance in peach, indicating the potential conservation of disease resistance genes at these loci.