Characterization of chromosome-specific S-SAP markers and their use in studying genetic diversity in Aegilops species

The short interspersed nuclear element (SINE), Au, was used to develop sequence-specific amplified polymorphism (S-SAP) markers for U- and M-genome chromosomes. The markers were localized using Triticum aestivum (wheat) - Aegilops geniculata and wheat - Aegilops biuncialis disomic chromosome additio...

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Published inGenome Vol. 49; no. 4; pp. 289 - 296
Main Authors Nagy, Ervin D, Molnár, István, Schneider, Annamária, Kovács, Géza, Molnár-Láng, Márta
Format Journal Article
LanguageEnglish
Published Ottawa, Canada NRC Research Press 01.04.2006
Canadian Science Publishing NRC Research Press
Subjects
Biological diversity
Chromosomes
Chromosomes, Plant - genetics
Deoxyribonucleic acid
DNA
DNA Fingerprinting
Genetic diversity
Genetic Markers
Genetic Speciation
Genetic Variation
Genome, Plant
Genomics
Nucleic Acid Amplification Techniques - methods
Phylogeny
Polymorphism
Polymorphism, Genetic
Retroelements
Sequence Analysis, DNA
Short Interspersed Nucleotide Elements - genetics
Triticum - genetics
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Summary:The short interspersed nuclear element (SINE), Au, was used to develop sequence-specific amplified polymorphism (S-SAP) markers for U- and M-genome chromosomes. The markers were localized using Triticum aestivum (wheat) - Aegilops geniculata and wheat - Aegilops biuncialis disomic chromosome addition lines. Thirty-seven markers distributed over 6 U and 6 M chromosomes were produced. A genetic diversity study carried out on 37 accessions from Ae. biuncialis, Ae. comosa, Ae. geniculata, and Ae. umbellulata suggested that Ae. biuncialis have arisen from its diploid ancestors more recently than Ae. geniculata. Several earlier studies indicated that the M genomes in polyploid Aegilops species had accumulated substantial rearrangements, whereas the U genomes remained essentially unmodified. However, this cannot be attributed to the preferential insertion of retroelements into the M genome chromosomes. Fourteen markers from a total of 8 chromosomes were sequenced; 3 markers were similar to known plant genes, 1 was derived from a long terminal repeat (LTR) retrotransposon, and 10 markers did not match to any known DNA sequences, suggesting that they were located in the highly variable intergenic regions.Key words: Aegilops, U and M genomes, S-SAP, genetic diversity, sequence characterization.
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ISSN:0831-2796
1480-3321
DOI:10.1139/g05-109