A dual organelle-targeting mechanosensitive probe
Cells are responsive to the mechanical environment, but the methods to detect simultaneously how different organelles react in mechanobiological processes remain largely unexplored. We herein report a dual organelle-targeting fluorescent probe, ( )-1-[3-(diethoxyphosphoryl)propyl]-4-[4-(diethylamino...
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Published in | Science advances Vol. 9; no. 2; p. eabn5390 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Association for the Advancement of Science
13.01.2023
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Subjects | |
Online Access | Get full text |
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Summary: | Cells are responsive to the mechanical environment, but the methods to detect simultaneously how different organelles react in mechanobiological processes remain largely unexplored. We herein report a dual organelle-targeting fluorescent probe, (
)-1-[3-(diethoxyphosphoryl)propyl]-4-[4-(diethylamino)styryl]pyridin-1-ium bromide (ASP-PE), for mechanical mapping in live cells. ASP-PE is aggregation-induced emission active and is sensitive to the local mechanical environment. It targets the plasma membrane (PM) and intracellular mitochondria in cells by its phosphonate moiety and pyridinium. In this work, through ASP-PE staining, changes of membrane tension in the PM and mitochondria in response to varied osmotic pressure and substrate stiffness are visualized using fluorescence lifetime imaging microscopy. The mechanobiological importance of actin filaments and microtubules in the PM and mitochondria is also investigated using this probe. Computational simulations are applied to study the sensing mechanism of the probe. This study introduces a unique tool for mapping the membrane tension in the PM and mitochondria together, providing us great opportunities to study organelle's interactions in mechanobiology. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Biological Inorganic Chemistry Laboratory, Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK. Present address: Department of Chemistry, King’s College London, 7 Trinity Street, London, SE1 1DB, UK. These authors contributed equally to this work. |
ISSN: | 2375-2548 2375-2548 |
DOI: | 10.1126/sciadv.abn5390 |