Transcription Factor OsbZIP60-like Regulating OsP5CS1 Gene and 2-Acetyl-1-pyrroline (2-AP) Biosynthesis in Aromatic Rice

• Published in: Plants (Basel) Vol. 13; no. 1; p. 49
• Main Authors: Bao, Gegen, Ashraf, Umair, Li, Lin, Qiao, Jingxuan, Wang, Chunling, Zheng, Yixiong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.12.2023; MDPI
• Subjects: 2-acetyl-1-pyrroline; Aroma; Baits; Biosynthesis; Chromosomes; CRISPR; Cultivars; Dehydrogenases; Enzymes; Fertilizers; fragrant rice; Gene expression; Gene regulation; Genes; Genetic transformation; Genomes; Homeostasis; Kinases; Mutants; Mutation; OsbZIP60-like; OsP5CS1; Overexpression; Proteins; Rice; Sorghum; Transcription activation; Transcription factors; Yeasts; OsP5CS1; OsbZIP60-like; fragrant rice; 2-acetyl-1-pyrroline
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13010049

The most important volatile in determining the aroma of fragrant rice is 2-Acetyl-1-pyrroline (2-AP); however, the transcriptional regulation mechanism of 2-AP biosynthesis in fragrant rice is still unclear. In this study, knockout mutant lines and over-expression lines were constructed by the genetic transformation of the Indica rice cultivar, i.e., 'Zhonghua11', which knocks out to produce fragrance in aromatic rice. The gene was also identified as a key gene in the 2-AP biosynthesis pathway of aromatic rice. The promoter was used as bait, and the OsbZIP60-like transcription factor was screened by yeast one-hybrid assays. The OsbZIP60-like transcription factor specifically bound to the gene. The dual luciferase reporting system found that the OsbZIP60-like transcription factor promoted the transcriptional activation of . Compared with the wild type, gene expression was significantly down-regulated in the -like mutant and resulted in a substantial reduction in 2-AP biosynthesis. Moreover, the gene expression was significantly up-regulated in -like over-expressed plants, and the 2-AP concentrations were also increased, whereas the -like mutants were found to be sensitive to Zn deficiency. Overall, the OsbZIP60-like transcription factor promoted the 2-AP accumulation. This study provides a theoretical basis for the transcriptional regulation mechanism of 2-AP biosynthesis and explores the function of the OsbZIP transcription factor in fragrant rice.