radish (Raphanus sativus L.) mitochondrial cox2 gene contains an ACG at the predicted translation initiation site

The mitochondrial cox2 gene has been sequenced from radish (Raphanus sativus L.). The gene is interrupted by a 1346-bp group-II intron and contains an ACG codon as the predicted translation initiation site. Analysis of cox2 cDNAs indicates that the ACG codon is not converted to an AUG codon in the m...

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Bibliographic Details
Published inCurrent genetics Vol. 34; no. 2; pp. 79 - 87
Main Authors Dong, F.G, Wilson, K.G, Makaroff, C.A
Format Journal Article
LanguageEnglish
Published United States 01.08.1998
Subjects
Amino Acid Sequence
amino acid sequences
Base Sequence
Brassica - enzymology
Brassica - genetics
Codon, Initiator - genetics
codons
complementary DNA
crops
cytochrome-c oxidase
DNA, Complementary - genetics
DNA, Mitochondrial - genetics
DNA, Plant - genetics
Electron Transport Complex IV - genetics
genbank/af036387
genbank/af036388
gene expression
Genes, Plant
initiation
introns
messenger RNA
mitochondrial DNA
Molecular Sequence Data
nucleotide sequences
Plant Proteins - genetics
Raphanus raphanistrum
Raphanus sativus
RNA Editing
RNA, Plant - genetics
RNA, Plant - metabolism
Sequence Homology, Amino Acid
species differences
translation
Vegetables - enzymology
Vegetables - genetics
Vegetables - metabolism
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Summary:The mitochondrial cox2 gene has been sequenced from radish (Raphanus sativus L.). The gene is interrupted by a 1346-bp group-II intron and contains an ACG codon as the predicted translation initiation site. Analysis of cox2 cDNAs indicates that the ACG codon is not converted to an AUG codon in the mRNA, although 15 other RNA editing sites were identified. The cox2 gene from Raphanus raphanistrum, and other varieties of R. sativus, also contain an ACG as the predicted start codon; plants in the closely related genus, Brassica, do not. Western-blot analyses indicate that cox2 proteins in radish mitochondria are the same size as those found in Brassica mitochondria and different from cox2 proteins in plants where cox2 is nuclear-encoded. This finding, along with the observation that cox2 sequences are not present in the nuclear genome of radish, suggests that ACG is utilized as the radish cox2 initiation codon.
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ISSN:0172-8083
1432-0983
DOI:10.1007/s002940050369