Chitosan beads as molecularly imprinted polymer matrix for selective separation of proteins

• Published in: Biomaterials Vol. 26; no. 28; pp. 5737 - 5745
• Main Authors: Guo, Tian-Ying, Xia, Yong-Qing, Wang, Jin, Song, Mou-Dao, Zhang, Bang-Hua
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.10.2005
• Subjects: Acrylic Resins - chemistry; Adsorption; Biocompatible Materials - chemistry; Chemical Fractionation - methods; Chitosan - chemistry; Chitosan beads; Chromatography - methods; Hemoglobins - chemistry; Hemoglobins - isolation & purification; Materials Testing; Microspheres; Molecular imprinting; Polyacrylamide; Porosity; Protein Binding; Protein separation; Serum Albumin, Bovine - chemistry; Serum Albumin, Bovine - isolation & purification; Surface Properties; Ultrafiltration - methods; Protein separation; Molecular imprinting; Polyacrylamide; Chitosan beads
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2005.02.017

Two kinds of molecularly imprinted polymers were prepared using hemoglobin as the imprinting molecule, acrylamide as the functional monomer, chitosan beads and maleic anhydride-modified chitosan beads as matrixes, respectively. Static adsorbing experimental results showed that an equal class of adsorption was formed in the imprinted polymers and the adsorption equilibrium constant and the maximum adsorption capacity were evaluated. Chromatographic characteristics showed that the column bedded with the hemoglobin imprinted beads could separate hemoglobin and bovine serum albumin effectively from their mixture, which indicates that the imprinted beads have very higher selectivity for hemoglobin than the non-imprinted with the same chemical composition.