The rapid and accurate determination of germ tube emergence site by Blumeria graminis conidia

• Published in: Physiological and molecular plant pathology Vol. 57; no. 6; pp. 281 - 301
• Main Authors: Wright, Alison J., Carver, Tim L.W., Thomas, Barry J., Fenwick, Nick I.D., Kunoh, Hitoshi, Nicholson, Ralph L.
• Format: Journal Article
• Language: English
• Published: London Elsevier India Pvt Ltd 01.12.2000; Elsevier
• Subjects: Avena sativa; Biological and medical sciences; Blumeria graminis; developmental regulation; Erysiphe graminis; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; germ tubes; germination; Hordeum vulgare; leaves; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; Phytopathology. Animal pests. Plant and forest protection; powdery mildew; recognition; Triticum aestivum; germination; Blumeria graminis; Avena sativa; powdery mildew; developmental regulation; Hordeum vulgare; recognition; Erysiphe graminis; Triticum aestivum; Site; Plant pathogen; Germinative tube; Rapid process; Determination; Spores; Fungi; Accurate position; Emergence; Ascomycetes; Thallophyta; Conidia
• ISSN: 0885-5765; 1096-1178
• DOI: 10.1006/pmpp.2000.0304

The development of appressoria by germinating Blumeria graminis conidia depends on its germ tubes making contact with the host surface. Low angle, low temperature scanning electron microscopy showed that 80% or more of first-formed germ tubes of f. spp. hordei, tritici and avenae conidia emerged from close to the host leaf surface, and so made contact with it allowing them to become functional primary germ tubes. Light microscopy of f. sp.hordei confirmed this result and, in addition, showed that germ tubes frequently emerged close to, and contacted, various hydrophobic and hydrophilic artificial substrata. Geometric models of conidium-substratum interfaces were developed and a “surface point method” was derived to predict the frequency with which contact would result if germ tube emergence was a random phenomenon. However, observed contact frequencies were far higher (c. three to eight times) than predicted. Thus, the germ tube emergence site was determined as a response to substratum contact. In part, this appeared to be a non-specific response. Nevertheless, germ tube contact frequencies were greater on the curved surface of leaf epidermal cells than on planar surface, suggesting that specific recognition of leaf surface characteristics may promote directional emergence. The area of contact required to stimulate directional germ tube emergence was very small: contact with a microneedle tip or with a spiders’ suspension thread was sufficient for many conidia. Similarly, on leaves, the only contact is between the tips of a limited number of conidial wall projections and the edges of epicuticular leaf wax plates. Micromanipulation to roll conidia so that their original site of contact with a leaf was rotated away from it, led to the majority of first-formed germ tubes growing away from the leaf, i.e. emerging close to the site of original contact. The experiments indicated that the site of germ tube emergence is determined within 1 min of deposition. This implicated the release of conidial extracellular materials in recognition of the conidium-leaf surface contact site.