Hypothalamic paraventricular nucleus stimulation reduces intestinal injury in rats with ulcerative colitis

• Published in: World journal of gastroenterology : WJG Vol. 22; no. 14; pp. 3769 - 3776
• Main Authors: Deng, Quan-Jun, Deng, Ding-Jing, Che, Jin, Zhao, Hai-Rong, Yu, Jun-Jie, Lu, Yong-Yu
• Format: Journal Article
• Language: English
• Published: United States Baishideng Publishing Group Inc 14.04.2016
• Subjects: Animals; Apoptosis - drug effects; Apoptosis Regulatory Proteins - metabolism; Basic Study; Colitis, Ulcerative - metabolism; Colitis, Ulcerative - pathology; Colitis, Ulcerative - physiopathology; Colitis, Ulcerative - prevention & control; colitis;Arginine; Colon - drug effects; Colon - innervation; Colon - metabolism; Colon - pathology; Cytoprotection; Disease Models, Animal; Dose-Response Relationship, Drug; Excitatory Amino Acid Agonists - pharmacology; Glutamic Acid - pharmacology; Inflammation Mediators - metabolism; nucleus;Nucleus; Oxidative Stress - drug effects; paraventricular; Paraventricular Hypothalamic Nucleus - drug effects; Paraventricular Hypothalamic Nucleus - metabolism; Paraventricular Hypothalamic Nucleus - physiopathology; Rats, Sprague-Dawley; Signal Transduction - drug effects; solitarius;Ulcerative; Solitary Nucleus - drug effects; Solitary Nucleus - physiopathology; tractus; Trinitrobenzenesulfonic Acid; Vagus Nerve - drug effects; Vagus Nerve - physiopathology; vasopressin;2,4,6-tri; Nucleus tractus solitarius; Paraventricular nucleus; Arginine vasopressin; 2,4,6-trinitrobenzene sulfonic acid; Ulcerative colitis
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v22.i14.3769

AIM: To investigate the effect and mechanism of stimulation of the hypothalamic paraventricular nucleus with glutamate acid in rats with ulcerative colitis(UC).METHODS: The rats were anesthetized with 10% chloral hydrate via abdominal injection and treated with an equal volume of TNBS + 50% ethanol enema, injected into the upper section of the anus with the tail facing up. Colonic damage scores were calculated after injecting a certain dose of glutamic acid into the paraventricular nucleus(p VN), and the effect of the nucleus tractus solitarius(NTS) and vagus nerve in alleviating UC injury through chemical stimulation of the p VN was observed in rats. Expression changes of C-myc, Apaf-1, caspase-3, interleukin(IL)-6, and IL-17 during the protection against UC injury through chemical stimulation of the p VN in rats were detected by Western blot. Malondialdehyde(MDA) content and superoxide dismutase(SOD) activity in colon tissues of rats were measured by colorimetric methods. RESULTS: Chemical stimulation of the PVN significantly reduced UC in rats in a dose-dependent manner. The protective effects of the chemical stimulationof the p VN on rats with UC were eliminated after chemical damage to the p VN. After glutamate receptor antagonist kynurenic acid was injected into the p VN, the protective effects of the chemical stimulation of the p VN were eliminated in rats with UC. After AVpVl receptor antagonist([Deamino-penl, val4, D-Arg8]-vasopressin) was injected into NTS or bilateral chemical damage to NTS, the protective effect of the chemical stimulation of p VN on UC was also eliminated. After chemical stimulation of the p VN, SOD activity increased, MDA content decreased, C-myc protein expression significantly increased, caspase-3 and Apaf-1 protein expression significantly decreased, and IL-6 and IL-17 expression decreased in colon tissues in rats with UC. CONCLUSION: Chemical stimulation of the hypothalamic p VN provides a protective effect against UC injury in rats. Hypothalamic p VN, NTS and vagus nerve play key roles in this process.