Determination of mycophenolic acid plasma levels in renal transplant recipients co-administered sirolimus: comparison of an enzyme multiplied immunoassay technique (EMIT) and liquid chromatography-tandem mass spectrometry

This study was designed to compare the enzyme multiplied immunoassay technique (EMIT) and a specific ;liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of mycophenolic acid (MPA) concentrations in plasma samples collected from renal transplant patients receiving...

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Bibliographic Details
Published inTherapeutic drug monitoring Vol. 28; no. 2; p. 274
Main Authors Prémaud, Aurélie, Rousseau, Annick, Picard, Nicolas, Marquet, Pierre
Format Journal Article
LanguageEnglish
Published United States 01.04.2006
Subjects
Area Under Curve
Chromatography, Liquid - methods
Creatinine - blood
Drug Monitoring - methods
Enzyme Inhibitors - blood
Enzyme Inhibitors - pharmacokinetics
Enzyme Inhibitors - therapeutic use
Enzyme Multiplied Immunoassay Technique
Graft Rejection - prevention & control
Humans
Kidney Transplantation
Mass Spectrometry - methods
Mycophenolic Acid - blood
Mycophenolic Acid - pharmacokinetics
Mycophenolic Acid - therapeutic use
Sirolimus - blood
Sirolimus - pharmacokinetics
Sirolimus - therapeutic use
Time Factors
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Summary:This study was designed to compare the enzyme multiplied immunoassay technique (EMIT) and a specific ;liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of mycophenolic acid (MPA) concentrations in plasma samples collected from renal transplant patients receiving mycophenolate mofetil in association with sirolimus and corticosteroids. Ten-point blood concentration-time profiles, between pre-dose (C0) and 9-hour post-dose, were obtained on day 7, day 14, months 1, 2, and 3 posttransplantation in 8 patients. MPA plasma concentrations were measured simultaneously with both EMIT and LC-MS/MS. Higher concentrations were obtained with EMIT. The mean (+/-SD) absolute difference was +0.84 +/- 1.93 mg/L, which corresponds to a significant overestimation of 18.7 +/- 2 6.8% with EMIT, with variations depending on the time elapsed since transplantation. An EMIT overestimation of 16.7 +/- 22.5% also was obtained for the MPA areas under the curve calculated using the trapezoidal rule.
ISSN:0163-4356
DOI:10.1097/01.ftd.0000197092.84935.ef