The succession of fecal bacterial community and its correlation with the changes of serum immune indicators in lambs from birth to 4 months

• Published in: Journal of Integrative Agriculture Vol. 22; no. 2; pp. 537 - 550
• Main Authors: YIN, Xue-jiao, JI, Shou-kun, DUAN, Chun-hui, TIAN, Pei-zhi, JU, Si-si, YAN, Hui, ZHANG, Ying-jie, LIU, Yue-qin
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.02.2023; College of Animal Science and Technology,Hebei Agricultural University,Baoding 071000,P.R.China; Elsevier
• Subjects: early life; establishment; fecal bacteria; serum parameter; sheep; establishment; fecal bacteria; serum parameter; sheep; early life
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/j.jia.2022.08.055

Early bacterial colonization and succession within the gastrointestinal tract have been suggested to be crucial in the development of host immunity. In this study, we have investigated the changes in live weight and concentrations of selected serum parameters in relation to their fecal bacterial communities as determined by high throughput sequencing of the 16S rRNA gene over the same period in lambs. The results showed that lambs’ growth performance, the serum parameters, fecal bacterial community and fecal bacterial functions were all affected (P<0.05) by age of the lambs. Similarity within age groups of fecal microbiota was lower in the preweaning period and increased sharply (P<0.05) after weaning at 60 days. The similarity between the samples collected from birth to 90 days of age and those collected at 120 days of age, increased (P<0.05) sharply after 30 days of age. Some age-associated changes in microbial genera were correlated with the changes in concentrations of immune indicators, including negative (P<0.05) correlations between the relative abundance of Lachnospiraceae UCG-010, Eubacterium coprostanoligenes group, Ruminococcaceae UCG-005, Ruminococcaceae UCG-009, Ruminococcaceae UCG-013, Ruminiclostridium 6, Ruminococcaceae UCG-008, and Oscillibacter with serum concentrations of lipopolysaccharide (LPS), D-lactate dehydrogenase (DLA), immunoglobulin (IgA, IgM, and IgG), and cytokines (interleukin-1β (IL-1β), IL-6, IL-12, and IL-17), tumor necrosis factor-α (TNF-α), and the relative abundance of these genera increased from 45 days of age. In conclusion, these results suggested that the age-related abundances of particular genera were correlated with serum markers of immunity in lambs, and there might be a critical window in the period from birth to 45 days of age which provide an opportunity for potential manipulation of the fecal microbial ecosystems to enhance immune function.