miRNA-155 Modulates the Malignant Biological Characteristics of NK/T-Cell Lymphoma Cells by Targeting FOXO3a Gene

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 6; pp. 882 - 888
• Main Author: 姬卫国 张旭东 孙向东 王祥麒 常保萍 张明智
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.12.2014; Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China%Department of Science and Research, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China%Department of Oncology, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China%Department of Hematology, The Second Affiliated Hospital of Henan University of Science and Technology, Luoyang 453000,China
• Subjects: Apoptosis - genetics; Forkhead Box Protein O3; Forkhead Transcription Factors - genetics; Forkhead Transcription Factors - metabolism; Gene Expression Regulation, Neoplastic; Humans; Jurkat Cells; Lentivirus; Lymphoma, T-Cell - genetics; Lymphoma, T-Cell - metabolism; Lymphoma, T-Cell - pathology; Medicine; Medicine & Public Health; MicroRNAs - biosynthesis; MicroRNAs - genetics; Natural Killer T-Cells - metabolism; Natural Killer T-Cells - pathology; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; RNA, Neoplasm - biosynthesis; RNA, Neoplasm - genetics; Transduction, Genetic; T细胞; Western印迹; 基因; 实时PCR; 恶性; 慢病毒载体; 淋巴瘤; 生物学特性; apoptosis; NK/T-cell lymphoma; FOXO3a; cell lines; miRNA-155; lentiviral vector
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1368-z

This study investigated the effects of miRNA-155 on malignant biological characteristics of NK/T-cell lymphoma cell lines and the possible mechanism. The expression of miRNA-155 was detected in lymphoma cell lines from different sources （SNK-6, YTS, Jurkat and DOHH2） by real-time PCR. Lentiviral vectors （pLL3.7） that could overexpress or downexpress miRNA-155 were constructed. Recombinant lentiviral particles were prepared and purified, and their titers determined. The expression of miRNA-155 in the infected SNK-6 cells and the cell proliferation were detected by PCR and CCK-8, respectively. Flow cytometry was used to determine the apoptosis of infected SNK-6 cells. The target of miRNA155 was predicted from Targetscan website. The effect of miRNA155 on FOXO3a expression was examined by Western blotting. The results showed that among the human NK/T-cell lymphoma cell lines SNK-6, YTS, Jurkat and DOHH2, the expression of miRNA-155 was highest in SNK-6. The infection efficiency of the recombinant lentivirns in SNK-6 was more than 70% at multiplicity of infection （MOI） of 100. The expression of miRNA-155 was significantly increased in SNK-6 cells infected by lentivirus vectors with high expression of miRNA-155 （4 times higher than the control group）, and profoundly decreased in those infected with lentivirnses with low expression of miRNA-155. The proliferation of letivirns-infected SNK-6 cells was decreased as the expression of miRNA-155 reduced. The apoptosis rate was increased with the reduction in the expression of miRNA-155. FOXO3a was found to be a possible target of miRNA155, as suggested by Targetscan website. Western blotting showed that the expression of FOXO3a was significantly elevated in SNK-6 cells with miRNA-155 inhibition. It was concluded that reduction in miRNA-155 expression can inhibit the proliferation of SNK-6 lymphoma cells and promote their apoptosis, which may be associated with regulation of FOXO3a gene.