IFN-γ Secretion in Gut of Ob/Ob Mice after Vertical Sleeve Gastrectomy and Its Function in Weight Loss Mechanism

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 36; no. 3; pp. 377 - 382
• Main Author: 杜锦鹏 汪赓 胡超杰 王清波 黎慧清 夏文芳 帅晓明 陶凯雄 王国斌 夏泽锋
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.06.2016; Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022.China
• Subjects: Animals; Body Weight; Cell Line; Gastrectomy - methods; Gene Expression; Hepatocytes - cytology; Hepatocytes - drug effects; Hepatocytes - metabolism; IFN-γ; Interferon-gamma - biosynthesis; Interferon-gamma - pharmacology; Interferon-gamma - secretion; Intestine, Small - drug effects; Intestine, Small - metabolism; Liver - drug effects; Liver - metabolism; Lymph Nodes - drug effects; Lymph Nodes - metabolism; Medicine; Medicine & Public Health; Mesentery - drug effects; Mesentery - metabolism; Mice; Mice, Obese; Obesity - metabolism; Obesity - pathology; Obesity - surgery; Receptors, Cytoplasmic and Nuclear - agonists; Receptors, Cytoplasmic and Nuclear - genetics; Receptors, Cytoplasmic and Nuclear - metabolism; Weight Loss; Western印迹法; 减肥; 分泌; 切除术; 垂直; 小鼠; 肠系膜淋巴结; weight loss; farnesoid X receptor; interferon-gamma; obesity; vertical sleeve gastrectomy
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-016-1595-6

Vertical sleeve gastrectomy（VSG） is becoming more and more popular among the world. Despite its dramatic efficacy, however, the mechanism of VSG remains largely undetermined. This study aimed to test interferon（IFN）-γ secretion n of mesenteric lymph nodes in obese mice（ob/ob mice）, a model of VSG, and its relationship with farnesoid X receptor（FXR） expression in the liver and small intestine, and to investigate the weight loss mechanism of VSG. The wild type（WT） mice and ob/ob mice were divided into four groups： A（WT＋Sham）, B（WT＋VSG）, C（ob/ob＋Sham）, and D（ob/ob＋VSG）. Body weight values were monitored. The IFN-γ expression in mesenteric lymph nodes of ob/ob mice pre- and post-operation was detected by flow cytometry（FCM）. The FXR expression in the liver and small intestine was detected by Western blotting. The mouse AML-12 liver cells were stimulated with IFN-γ at different concentrations in vitro. The changes of FXR expression were also examined. The results showed that the body weight of ob/ob mice was significantly declined from（40.6±2.7） g to（27.5±3.8） g on the 30 th day after VSG（P〈0.05）. At the same time, VSG induced a higher level secretion of IFN-γ in mesenteric lymph nodes of ob/ob mice than that pre-operation（P〈0.05）. The FXR expression levels in the liver and small intestine after VSG were respectively 0.97±0.07 and 0.84±0.07 fold of GAPDH, which were significantly higher than pre-operative levels of 0.50±0.06 and 0.48±0.06 respectively（P〈0.05）. After the stimulation of AML-12 liver cells in vitro by different concentrations of IFN-γ（0, 10, 25, 50, 100, and 200 ng/m L）, the relative FXR expression levels were 0.22±0.04, 0.31±0.04, 0.39±0.05, 0.38±0.05, 0.56±0.06, and 0.35±0.05, respectively, suggesting IFN-γ could distinctly promote the FXR expression in a dose-dependent manner in comparison to those cells without IFN-γ stimulation（P〈0.05）. It was concluded that VSG induces a weight loss in ob/ob mice by increasing IFN-γ secretion of mesenteric lymph nodes, which then increases the FXR expression of the liver and small intestine.