Fluorescent Toolbox for Assessing Protein Location and Function
Advances in molecular biology, organic chemistry, and materials science have recently created several new classes of fluorescent probes for imaging in cell biology. Here we review the characteristic benefits and limitations of fluorescent probes to study proteins. The focus is on protein detection i...
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Published in | Science (American Association for the Advancement of Science) Vol. 312; no. 5771; pp. 217 - 224 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Association for the Advancement of Science
14.04.2006
The American Association for the Advancement of Science |
Subjects | |
Online Access | Get full text |
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Summary: | Advances in molecular biology, organic chemistry, and materials science have recently created several new classes of fluorescent probes for imaging in cell biology. Here we review the characteristic benefits and limitations of fluorescent probes to study proteins. The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light microscopy with electron microscopy. Small organic fluorescent dyes, nanocrystals ("quantum dots"), autofluorescent proteins, small genetic encoded tags that can be complexed with fluorochromes, and combinations of these probes are highlighted. |
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Bibliography: | http://www.scienceonline.org/ ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 ObjectType-Review-3 content type line 23 ObjectType-Feature-3 ObjectType-Review-1 |
ISSN: | 0036-8075 1095-9203 |
DOI: | 10.1126/science.1124618 |