Production of live larvae following in vitro maturation of zebrafish oocytes

• Published in: Theriogenology Vol. 105; pp. 115 - 119
• Main Authors: Silva, Laura A., Marques, Lis S., Zhang, Tiantian, Rodrigues, Rômulo B., Eloy, Lidiane Raquel, Streit, Danilo P.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2018
• Subjects: Animals; Carp pituitary; Danio rerio; Dose-Response Relationship, Drug; In Vitro Oocyte Maturation Techniques - veterinary; In vitro maturation; Larva - physiology; Oocytes - drug effects; Oocytes - growth & development; Ovarian follicles; Pituitary Gland - chemistry; Pituitary gonadotropins; Tissue Extracts - administration & dosage; Tissue Extracts - chemistry; Tissue Extracts - pharmacology; Zebrafish - physiology; Pituitary gonadotropins; Carp pituitary; Ovarian follicles; Danio rerio; In vitro maturation
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2017.09.021

This study aimed to assess the effects of carp pituitary extract (CPE), follicle stimulating hormone (FSH) and luteinizing hormone (LH) on zebrafish oocyte maturation and the ability of these mature oocytes to be fertilized and developed until hatching. Stage III follicles were matured in eight treatments: five concentrations of CPE (16, 32, 48, 64 and 80 μg/mL), one of FSH (0.5 μg/mL), one of LH (0.5 μg/mL), or one combination of FSH (0.5 μg/mL) and LH (0.5 μg/mL). Maturation rates in CPE treatments were 12.8% (16 μg/mL), 24.8% (32 μg/mL), 27.0% (48 μg/mL), 22.7% (64 μg/mL) and 9.6% (80 μg/mL); in FSH was 15.7% (0.5 μg/mL), in LH was 31.8% (0.5 μg/mL) and in FSH (0.5 μg/mL) combined with LH (0.5 μg/mL) it was 50.4%. In vitro fertilization was performed in all treatments; however, only the treatment combining FSH and LH resulted in fertilized oocytes. After maturation using FSH combined with LH, the cleavage rate was 33.3% and hatching rate of live larvae was 20.0%. These results showed that FSH combined with LH was effective in IVM of zebrafish oocyte. •FSH combined with LH is effective on IVM of zebrafish oocyte.•FSH combined with LH may be applied to IVM of other teleost.•Pituitary concentration used in zebrafish IVM was dose-dependent.•FSH combined with LH result in zebrafish live larvae after IVF.