Molecular cloning and characterization of canine ICOS

• Published in: Genomics (San Diego, Calif.) Vol. 84; no. 4; pp. 730 - 736
• Main Authors: Lee, Je-Hwan, Joo, Young-Don, Yim, Daesong, Lee, Richard, Ostrander, Elaine A., Loretz, Carol, Little, Marie-Térèse, Storb, Rainer, Kuhr, Christian S.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.10.2004; Elsevier
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; Antigens, Differentiation, T-Lymphocyte - genetics; Antigens, Differentiation, T-Lymphocyte - metabolism; Base Sequence; Biological and medical sciences; Canine; Cell receptors; Cell structures and functions; Chromosome Mapping; Chromosomes - genetics; Cloning; Cloning, Molecular; complementary DNA; Costimulation; Dogs; Fundamental and applied biological sciences. Psychology; Gene; Genes. Genome; Genetics of eukaryotes. Biological and molecular evolution; Humans; ICOS; Immunity; Inducible; inducible costimulatory receptor; Inducible T-Cell Co-Stimulator Protein; leukocytes; Leukocytes - metabolism; Mice; Miscellaneous; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; nucleotide sequences; Open Reading Frames; Receptor; receptors; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Transplantation; Inducible; Gene; Costimulation; Receptor; Cloning; Transplantation; ICOS; Immunity; Canine; Fissipedia; Carnivora; Genomics; Characterization; Vertebrata; Mammalia; Molecular cloning; Dog; Biological receptor
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2004.06.009

Inducible costimulatory receptor (ICOS) is one recently identified member of the CD28 family of costimulatory molecules. Evidence suggests ICOS functions as a critical immune regulator and, to evaluate these effects, we employed the canine model system that has been used to develop strategies currently in clinical use for hematopoietic stem cell transplantation. To investigate the effects of blocking the ICOS pathway in the canine hematopoietic cell transplantation model, we tested existing murine and human reagents and cloned the full length of the open reading frame of canine ICOS cDNA to allow the development of reagents specific for the canine ICOS. Canine ICOS contains a major open reading frame of 624 nucleotides, encoding a protein of 208 amino acids, and localizes to chromosome 37. Canine ICOS shares 79% sequence identity with human ICOS, 70% with mouse, and 69% with rat. Canine ICOS expression is limited to stimulated PBMC.