Phenylalkyl Glycosides from the Flowers of Brugmansia arborea L. and Their Radical Scavenging Effect and Protective Effect on Pancreatic Islets Damaged by Alloxan in Zebrafish ( Danio rerio ) Larvae
The study aimed to investigate the antioxidant and antidiabetic activity of L. flower extracts, solvent fractions, and isolated compounds. L flowers were extracted with aqueous methanol, and concentrated extract was successively partitioned into EtOAc, -BuOH, and H O fractions. Repeated silica gel a...
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Published in | Plants (Basel) Vol. 12; no. 24; p. 4075 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
05.12.2023
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | The study aimed to investigate the antioxidant and antidiabetic activity of
L. flower extracts, solvent fractions, and isolated compounds.
L flowers were extracted with aqueous methanol, and concentrated extract was successively partitioned into EtOAc,
-BuOH, and H
O fractions. Repeated silica gel and octadecyl silica gel column chromatographies for EtOAc and
-BuOH fractions led to the isolation of a new phenylalkyl glycoside (
), along with five known ones. Several spectroscopic data led to the structure determination of one new phenylalky glycoside as brugmansioside C (named) (
) and five known ones as benzyl-
-
-D-glucopyranoside (
), benzyl-
-
-D-glucosyl-(1→6)-
-D-glucopyranoside (
), 2-phenylethyl-
-
-D-glucopyranoside (
), 2-phenylethyl-
-
-D-glucosyl-(1→6)-
-D-glucopyranoside (
), and 3-phenylpropyl-
-
-D-glucopyranoside (
). The five known ones (
-
) were isolated from
flowers for the first time in this study. The extract, solvent fractions, and all isolated compounds showed radical scavenging activities using ABTS radical, and EtOAc fraction showed the highest scavenging capacity, whereas compounds 2, 4, and 6 did not display the capacity to use the DPPH radical. The extract, solvent fractions, and all isolated compounds showed a protective effect on pancreatic islets damaged by alloxan treatment in zebrafish larvae. The pancreatic islet size treated with EtOAc,
-BuOH fractions, and all compounds significantly increased by 64.0%, 69.4%, 82.0%, 89.8%, 80.0%, 97.8%, 103.1%, and 99.6%, respectively, compared to the alloxan-induced group. These results indicate that
flowers and their isolated compounds are useful as potential antioxidant and antidiabetic agents. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2223-7747 2223-7747 |
DOI: | 10.3390/plants12244075 |