Effect of the consumption of hesperidin in orange juice on the transcriptomic profile of subjects with elevated blood pressure and stage 1 hypertension: A randomized controlled trial (CITRUS study)

• Published in: Clinical nutrition (Edinburgh, Scotland) Vol. 40; no. 12; pp. 5812 - 5822
• Main Authors: Pla-Pagà, Laura, Valls, Rosa M., Pedret, Anna, Calderón-Pérez, Lorena, Llauradó, Elisabet, Companys, Judit, Domenech-Coca, Cristina, Canela, Nuria, del Bas, Josep M., Caimari, Antoni, Puiggròs, Francesc, MI, Covas, Arola, Lluís, Solà, Rosa
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2021
• Subjects: 3-Hydroxysteroid Dehydrogenases - metabolism; Adult; Blood Pressure - genetics; Citrus; Citrus sinensis; clinical nutrition; Double-Blind Method; Down-Regulation; Female; Fruit and Vegetable Juices; Gene Expression - drug effects; genes; Hesperidin; Hesperidin - administration & dosage; Hesperidin - pharmacology; Humans; hypertension; Hypertension - genetics; Hypertensive subjects; inflammation; insulin resistance; Leukocytes, Mononuclear - metabolism; Male; mechanism of action; Middle Aged; Orange juice; PBMCs; Plasma; randomized clinical trials; Selenium-Binding Proteins - metabolism; systolic blood pressure; Transcriptome - drug effects; Transcriptomic analysis; transcriptomics; SBP; Plasma; CD; EOJ; LncRNA; Transcriptomic analysis; PBMCs; CVD; Hypertensive subjects; DBP; Orange juice; IPA; OJ; Hesperidin; CVDR; CVDRFs
• ISSN: 0261-5614; 1532-1983; 1532-1983
• DOI: 10.1016/j.clnu.2021.10.009

Hesperidin exerts cardiovascular beneficial effects, but its mechanisms of action remain undefined. In a previous study we demonstrated that a single dose and a 12-week treatment of hesperidin decreased systolic blood pressure. The aim of this study was to ascertain the action mechanisms of hesperidin consumption in subjects with elevated blood pressure or with stage 1 hypertension, by determining their transcriptomic profile after a single dose or a 12-week treatment. For transcriptomic analysis, peripheral blood mononuclear cells were obtained from 37 subjects with elevated blood pressure and stage 1 hypertension from CITRUS study who were randomized to receive for 12 weeks: control drink (CD; n = 11), OJ (containing 345 mg of hesperidin; n = 15) or EOJ (containing 600 mg of hesperidin; n = 11). Before starting the 12-weeks treatment, a single dose study with a 6 h of follow-up in each group was performed. After the single dose consumption, EOJ versus OJ, downregulated DHRS9 gene which is related with insulin resistance. Compared to CD, 12-week treatment of EOJ downregulated 6 proinflammatory genes while after OJ consumption only 1 proinflammatory gene was downregulated. Moreover, 12-week treatment of EOJ versus OJ, downregulated acute coronary syndrome gene related (SELENBP1). A single dose consumption of EOJ could protect from insulin resistance. Moreover, EOJ decrease the expression of proinflammatory genes after 12-week treatment providing a possible mechanism of action on inflammation pathway.