OsCSD2 and OsCSD3 Enhance Seed Storability by Modulating Antioxidant Enzymes and Abscisic Acid in Rice

• Published in: Plants (Basel) Vol. 13; no. 2; p. 310
• Main Authors: Zheng, Xiaohai, Yuan, Zhiyang, Yu, Yuye, Yu, Sibin, He, Hanzi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.01.2024; MDPI
• Subjects: Abscisic acid; Aging; antioxidant capacity; Antioxidants; Association analysis; Cell membranes; Chloroplasts; Chromosomes; CRISPR; Crop production; Crop yield; Electrical conductivity; Electrical resistivity; Enzymes; Food security; Genes; Genomes; Germplasm; High temperature; Humidity; Oxidative stress; phytohormone; Proteins; Regulatory mechanisms (biology); Rice; seed storability; Seeds; Signal transduction; superoxidase; Superoxide dismutase; antioxidant capacity; seed storability; superoxidase; phytohormone
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13020310

Seed deterioration during storage poses a significant challenge to rice production, leading to a drastic decline in both edible quality and viability, thereby impacting overall crop yield. This study aimed to address this issue by further investigating candidate genes associated with two previously identified QTLs for seed storability through genome association analysis. Among the screened genes, two ( ) genes, ( ) and , were selected for further study. The generation of overexpression and CRISPR/Cas9 mutant transgenic lines revealed that and play a positive regulatory role in enhancing rice seed storability. Subsequent exploration of the physiological mechanisms demonstrated that overexpression lines exhibited lower relative electrical conductivity, indicative of reduced cell membrane damage, while knockout lines displayed the opposite trend. Furthermore, the overexpression lines of and showed significant increases not only in SOD but also in CAT and POD activities, highlighting an augmented antioxidant system in the transgenic seeds. Additionally, hormone profiling indicated that ABA contributed to the improved seed storability observed in these lines. In summary, these findings provide valuable insights into the regulatory mechanisms of in rice storability, with potential applications for mitigating grain loss and enhancing global food security.