Muscle creatine kinase isoenzyme expression in adult human brain
Previous studies have suggested that MM creatine kinase is a muscle-specific protein and is not present in adult brain tissue. We have isolated a protein from human brain with an apparent molecular weight of 43,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis which is i...
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Published in | The Journal of biological chemistry Vol. 265; no. 11; pp. 6403 - 6409 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
American Society for Biochemistry and Molecular Biology
15.04.1990
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Subjects | |
Online Access | Get full text |
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Summary: | Previous studies have suggested that MM creatine kinase is a muscle-specific protein and is not present in adult brain tissue.
We have isolated a protein from human brain with an apparent molecular weight of 43,000 as determined by sodium dodecyl sulfate-polyacrylamide
gel electrophoresis which is identical to the muscle M creatine kinase isoenzyme subunit at all 30 sequenced amino acid residues
and possesses creatine kinase enzymatic activity following nondenaturing agarose-gel electrophoresis. Immunohistochemistry
localizes M creatine kinase to discrete areas of adult human brain. Northern blot analysis of both total and poly(A)-selected
RNA isolated from brain did not detect M creatine kinase mRNA. However, polymerase chain reaction amplification of cDNA synthesized
from human placenta, heart, and brain mRNA detected M creatine kinase message in both heart and brain but not placenta which
contains no detectable M creatine kinase protein. N1E115 and NS20Y, mouse neuroblastoma cell lines which have been used as
models of neural cell differentiation, were found also to express MM creatine kinase. Moreover, a transiently transfected
reporter gene with 4,800 base pairs of M creatine kinase upstream region fused to chloramphenicol acetyltransferase was expressed
during differentiation of these neural cell lines. In summary, MM creatine kinase is present in human brain and we suggest
the M creatine kinase upstream region is sufficient to modulate M creatine kinase expression in certain neuronal cells and
may be regulated independently from other muscle genes. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(19)39340-8 |