Establishment of Reference Intervals and Transfusion Criterion for Sonoclot Analysis

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 36; no. 4; pp. 614 - 617
• Main Author: 张真路 陈佑平 陶翠华 刘晓辉 李梦雅 周新
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.08.2016; Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China%Department of Pathology,Wuhan Asia Heart Hospital of Wuhan University, Wuhan 430022, China%Department of Transfusion,Wuhan Asia Heart Hospital of Wuhan University, Wuhan 430022, China%Department of Laboratory Medicine,Wuhan Asia Heart Hospital of Wuhan University, Wuhan 430022, China%Department of Cardiology, Wuhan Asia Heart Hospital of Wuhan University, Wuhan 430022, China
• Subjects: Adolescent; Adult; Aged; APTT; Blood Coagulation; Cardiopulmonary Bypass; China; Female; Fibrinogen - metabolism; Humans; Male; Medicine; Medicine & Public Health; Middle Aged; Partial Thromboplastin Time - methods; Platelet Count; Point-of-Care Systems; Reference Values; 区间; 工作特性曲线; 时间间隔; 标准; 活化部分凝血活酶时间; 血小板功能; 输血; transfusion criterion; reference interval; Sonoclot analyzer
• ISSN: 1672-0733; 1993-1352; 1993-1352
• DOI: 10.1007/s11596-016-1634-3

Sonoclot analyzer has been widely used in many countries. But the reference intervals provided by the manufacturer were derived from only 45 participants, and there was no cut-off value for transfusion for Sonoclot analysis. This study aimed to establish reference intervals and transfusion criterion for Sonoclot analysis. Volunteers were recruited from healthy Chinese adults and patients undergoing cardiac surgery. Blood samples were withdrawn from forearm vein and measured for activated clotting time（ACT）, clot rate（CR）, platelet function（PF）, activated partial thromboplastin time（APTT）, fibrinogen concentration（FIB）, and platelet count（PLT）. The reference intervals were determined by the nonparametric method. Cut-off values were determined by the receiver operating characteristics curve. A total of 135 healthy volunteers and 281 patients were enrolled. The 95% reference intervals were 96–195 s, 22–51 signal U/min, 〉1.6 for ACT, CR, PF respectively. In the 281 patients, the results of APTT, FIB, PLT, ACT, CR, and PF ranged from 20.5–300.0 s, 0.28–4.11 g/L,（19.0–387.3）×109/L, 80–514 s, 2.9–74 signal U/min, and 0.1–5.1 respectively. The cut-off values for transfusion were 〉208, ≤14, and ≤1.3 for ACT, CR, PF respectively. The cut-off values of Sonoclot analysis were within the manufacturer＇s reference intervals, while they were outside the reference intervals established in this study. The results suggested that the manufacturer＇s reference intervals were not suitable for Chinese. The reference intervals and cut-off values established in this study will be helpful to Chinese patients.