A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic

• Published in: Food and chemical toxicology Vol. 62; pp. 427 - 431
• Main Authors: Dohnal, Vlastimil, Dvořák, Vladimír, Malíř, František, Ostrý, Vladimír, Roubal, Tomáš
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.12.2013; Elsevier
• Subjects: Adult; adverse effects; Biological and medical sciences; blood serum; Calibration; carcinogens; Chromatography, High Pressure Liquid - methods; Czech Republic; detection limit; ELISA; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Female; genotoxicity; Germany; high performance liquid chromatography; HPLC; human health; Humans; Limit of Detection; Medical sciences; methanol; Ochratoxin A; Ochratoxins - blood; parenting; patients; Plant poisons toxicology; Reproducibility of Results; risk; Serum; Toxicology; women; Young Adult; Czech Republic; Europe; Germany; Serum; Ochratoxin A; HPLC; ELISA; Human; Biological fluid; Ochratoxin; Mycotoxin; HPLC chromatography; Enzyme immunoassay; Blood; Assay; Toxin; ELISA assay; Comparative study; Quantitative analysis
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2013.09.010

•First monitoring of ochratoxin A in pregnant population in Czech Republic.•Comparison of two analytical methods and discussion of their limits at low ochratoxin A concentrations.•Comparison of results between population in Czech Republic and Germany. Ochratoxin A (OTA) is one of the most naturally occurring fungal toxins in food. It has been detected in high concentrations in serum samples of nephropathic patients and can be applied as one of the markers of potential risk of this disease. Also, OTA can cause adverse effects on human health such as genotoxicity and is anticipated to be a potential human carcinogen. In this study, enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) were applied in analysis of 115 blood serum samples of women in the child rearing age from the Czech Republic and both methods were compared. The OTA was presented in a broad range of concentrations from 0.037 to 1.130μg/L. The outcome of ELISA and HPLC measurements were well correlated (r=0.907). However, it was observed that ELISA tend to result in underestimating the OTA level at the low serum concentrations. Both methods had the same limits of quantification of 0.050μg/L under standard operation conditions. When OTA concentration in a sample was too low, the sample was redissolved in only 300μL of methanol and the detection limit for HPLC was lowered to 0.030μg OTA/L.