Self-assembled gel tubes, filaments and 3D-printing with in situ metal nanoparticle formation and enhanced stem cell growth

• Published in: Chemical science (Cambridge) Vol. 13; no. 7; pp. 1972 - 1981
• Main Authors: Piras, Carmen C, Kay, Alasdair G, Genever, Paul G, Fitremann, Juliette, Smith, David K
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 16.02.2022; The Royal Society of Chemistry
• Subjects: Biocompatibility; Calcium alginate; Chemical Sciences; Chemistry; Filaments; Gels; Gold; Low molecular weights; Nanoparticles; Reduction (metal working); Self-assembly; Stem cells; Three dimensional printing; Tissue engineering; Toxicity; Tubes; Wet spinning
• ISSN: 2041-6520; 2041-6539
• DOI: 10.1039/d1sc06062g

This paper reports simple strategies to fabricate self-assembled artificial tubular and filamentous systems from a low molecular weight gelator (LMWG). In the first strategy, tubular 'core-shell' gel structures based on the dibenzylidenesorbitol-based LMWG DBS-CONHNH were made in combination with the polymer gelator (PG) calcium alginate. In the second approach, gel filaments based on DBS-CONHNH alone were prepared by wet spinning at elevated concentrations using a 'solvent-switch' approach. The higher concentrations used in wet-spinning prevent the need for a supporting PG. Furthermore, this can be extended into a 3D-printing method, with the printed LMWG objects showing excellent stability for at least a week in water. The LMWG retains its unique ability for precious metal reduction, yielding Au nanoparticles (AuNPs) within the tubes and filaments when they are exposed to AuCl solutions. Since the gel filaments have a higher loading of DBS-CONHNH , they can be loaded with significantly more AuNPs. Cytotoxicity and viability studies on human mesenchymal stem cells show that the DBS-CONHNH and DBS-CONHNH /alginate hybrid gels loaded with AuNPs are biocompatible, with the presence of AuNPs enhancing stem cell metabolism. Taken together, these results indicate that DBS-CONHNH can be shaped and 3D-printed, and has considerable potential for use in tissue engineering applications.