Development and application of KASP marker for high throughput detection of the seedless trait in grapevine

• Published in: Journal of Integrative Agriculture Vol. 22; no. 11; pp. 3269 - 3283
• Main Authors: WANG, Fu-qiang, BIAN, Lu, QIU, Peng-peng, GUO, Shuo, GUO, Jing-han, GUO, Chen-shuo, JIANG, Jian-fu, LIU, Chong-huai, WANG, Yong, LIU, Guo-tian, WANG, Yue-jin, XU, Yan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2023; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China,Ministry of Agriculture and Rural Affairs/State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/College of Horticulture,Northwest A&F University,Yangling 712100,P.R.China%Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,P.R.China%Research Institute of Grape and Melon of Xinjiang Uygur Autonomous Region,Shanshan 838200,P.R.China; Elsevier
• Subjects: KASP; MAS; seedless grape; SSR; KASP; SSR; MAS; seedless grape
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/j.jia.2023.10.014

Molecular marker-assisted selection (MAS) can significantly accelerate and improve the efficiency of the breeding process in seedless grape cultivars. In this study, we developed the KASP_VviAGL11 and VviAGL11_410 markers based on a single nucleotide polymorphism (SNP) site (Chr18: 26889437 (A/C)) of the VviAGL11 gene, and compared them with previously reported SSR markers p3_VvAGL11 and 5U_VviAGL11 by testing 101 cultivars and 81 F1 hybrid progenies. The results showed that both of the proposed markers obtained 100% accuracy rates in detecting allele A, which was closely associated with the seedless trait in grapes, while p3_VvAGL11 and 5U_VviAGL11 had lower accuracy rates due to their tendency to produce false positives. After careful evaluation of the technical advantages and disadvantages associated with these markers, we concluded that KASP_VviAGL11 was superior in terms of simplicity, cost-effectiveness, efficiency, and accuracy. Thus, we optimized the process of molecular MAS for seedless grapes, focusing on the KASP_VviAGL11 marker as a central component, to provide key technical support for the development of new seedless grape cultivars.