Repeated high-intensity Wingate cycle bouts influence markers of lymphocyte migration but not apoptosis

• Published in: Applied physiology, nutrition, and metabolism Vol. 37; no. 2; pp. 241 - 246
• Main Authors: Friedman, Rachel A, Navalta, James W, Fedor, Elizabeth A, Kell, Holly B, Lyons, T Scott, Arnett, Scott W, Schafer, Mark A
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.04.2012; Canadian Science Publishing NRC Research Press
• Subjects: Adult; Annexin A5 - metabolism; Apoptosis; Apoptosis - physiology; Biomarkers; CD4-Positive T-Lymphocytes - metabolism; CD8-Positive T-Lymphocytes - metabolism; Cell adhesion & migration; Cell cycle; Cell death; Cell Movement; Cells; exercice répété; Exercise - physiology; Female; Humans; leucocytes; leukocytes; Lymphocyte Count; Lymphocyte Subsets - cytology; Lymphocyte Subsets - physiology; Lymphocytes; Male; mort cellulaire programmée; Oxygen Consumption; programmed cell death; repetitive exercise; Wingate anaerobic cycle test; Young Adult; épreuve anaérobie de Wingate sur ergocycle; United States
• ISSN: 1715-5312; 1715-5320
• DOI: 10.1139/h11-156

Studies have shown significant changes in lymphocytes during continuous exercise, but little has been shown on the effect of repeated high intensity bouts. This study was designed to examine the effect of repeated intermittent bouts on lymphocyte subset cell count, apoptosis, and migration. A series of 6 Wingate anaerobic cycle tests were performed by participants (N = 8) with blood samples attained before, immediately following, and after a designated recovery period (excess postexercise oxygen consumption (EPOC)) to observe lymphocyte changes. Lymphocyte subsets (CD4+, CD4/CD45RA+, CD8+, CD8+/CD45RA+, CD19+) were assessed for apoptosis (annexin V+) and cellular migration (CX 3 CR1). Our results indicate that the CD8+ and CD8+/CD45RA+ subsets were significantly influenced by the repetitive Wingate cycling protocol such that cell counts increased with exercise, and then decreased at EPOC termination (p = 0.016). The observed postexercise decrease in CD8+ and CD8+/CD45RA+ cells was accompanied by a significant change in the CX 3 CR1 cell migration receptor (p = 0.019), but not apoptosis (p = 0.87). This indicates that with repetitive high-intensity cycling, the response in CD8+ cells following the bout is likely due to cell migration rather than cell death.