Circulating inflammatory miRNA signature in response to different doses of aerobic exercise

• Published in: Journal of applied physiology (1985) Vol. 119; no. 2; pp. 124 - 134
• Main Authors: de Gonzalo-Calvo, David, Dávalos, Alberto, Montero, Ana, García-González, Ángela, Tyshkovska, Iryna, González-Medina, Antonio, Soares, Sara M A, Martínez-Camblor, Pablo, Casas-Agustench, Patricia, Rabadán, Manuel, Díaz-Martínez, Ángel E, Úbeda, Natalia, Iglesias-Gutiérrez, Eduardo
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 15.07.2015
• Subjects: Adult; Biomarkers - blood; Exercise; Exercise - physiology; Humans; Inflammation - blood; Male; Men; MicroRNAs - blood; Middle age; Ribonucleic acid; RNA; Running - physiology; inflammation; exercise; circulating microRNAs
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.00077.2015

While moderate acute exercise has been associated with strong anti-inflammatory mechanisms, strenuous exercise has been linked to deleterious inflammatory perturbations. It is therefore fundamental to elucidate the mechanisms that regulate the exercise-induced inflammatory cascade. Information on novel regulators such as circulating inflammatory microRNAs (c-inflammamiRs) is incomplete. In this study, we evaluated the response of a panel of c-inflammamiRs to different doses of acute aerobic exercise. We first studied the exercise-induced inflammatory cascade in serum samples of nine active middle-aged males immediately before and after (0 h, 24 h, 72 h) 10-km, half-marathon, and marathon races. Next, we analyzed the circulating profile of 106 specific c-inflammamiRs immediately before) and after (0 h, 24 h) 10-km (low inflammatory response) and marathon (high inflammatory response) races. Analysis of classical inflammatory parameters revealed a dose-dependent effect of aerobic exercise on systemic inflammation, with higher levels detected after marathon. We observed an increase in miR-150-5p immediately after the 10-km race. Levels of 12 c-inflammamiRs were increased immediately after the marathon (let-7d-3p, let-7f-2-3p, miR-125b-5p, miR-132-3p, miR-143-3p, miR-148a-3p, miR-223-3p, miR-223-5p, miR-29a-3p, miR-34a-5p, miR-424-3p, and miR-424-5p). c-inflammamiRs returned to basal levels after 24 h. Correlation and in silico analyses supported a close association between the observed c-inflammamiR pattern and regulation of the inflammatory process. In conclusion, we found that different doses of acute aerobic exercise induced a distinct and specific c-inflammamiR response, which may be associated with control of the exercise-induced inflammatory cascade. Our findings point to c-inflammamiRs as potential biomarkers of exercise-induced inflammation, and hence, exercise dose.