Separation of Urea, Uric Acid, Creatine, and Creatinine by Micellar Electrokinetic Capillary Chromatography with Sodium Cholate

• Published in: Journal of chromatographic science Vol. 37; no. 2; pp. 45 - 50
• Main Authors: Yan, Sheng-Lei, Lin, Pei-Zung, Hsiao, Meen-Woon
• Format: Journal Article
• Language: English
• Published: Niles, IL Oxford University Press 01.02.1999; Preston Publications
• Subjects: Analytical chemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Micellar Electrokinetic Capillary - methods; Creatine - isolation & purification; Creatinine - isolation & purification; Exact sciences and technology; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Other chromatographic methods; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Sodium Cholate - chemistry; Temperature; Urea - isolation & purification; Uric Acid - isolation & purification; Urinary system; Creatinine; Chemical analysis; Renal function; Micellar electrokinetic capillary chromatography; Temperature effect; Retention factor; Uric acid; Creatine; Experimental study; Urea; Ultraviolet detector; Analysis method; Electrophoretic mobility; Clinical biology; Monitoring
• ISSN: 0021-9665; 1945-239X
• DOI: 10.1093/chromsci/37.2.45

The capillary electrophoretic separation of the four nonprotein nitrogenous compounds (NPNs; urea, uric acid, creatine, and creatinine) typically employed in clinical and medical settings for the monitoring of renal function is described. Successful resolution of these compounds is achieved with the use of a bile salt micelle system composed of sodium cholate at phosphate buffer pH 7.4. The elution patterns of four NPNs are obtained within 30 min with a voltage of 30 kV. The effect of varying the applied voltage, temperature, and the mole ratio of phosphate buffer with bile salt surfactant on the migration behavior is also examined.